{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:13:01Z","timestamp":1773306781582,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":2040,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Dutch Organization for Scientific Research","award":["900-506-160"],"award-info":[{"award-number":["900-506-160"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:p>Cyclosporin A (CsA), a potent inhibitor of T\u2010cell activation, has been shown to have several\neffects on thymocyte maturation, thymic stromal cells, and the generation of autoreactive T\ncells. In Lewis rats, the combination of lethal irradiation, syngeneic bone marrow\ntransplantation, and a 4\u2010week course of CsA administration causes the development of an\nautoimmune disease (CsA\u2010AI) resembling allogeneic graft\u2010versus\u2010host disease. This occurs\nupon withdrawal of CsA, provided the thymus receives irradiation and is present during\nCsA treatment. In this study, the separate effects of irradiation or CsA treatment on thymic\nstromal cells and thymocytes, compared to the combinatory effects, were examined using\nimmunohistochemistry and tricolor flow cytometric analysis.<\/jats:p><jats:p>CsA treatment causes an involution of the thymic medulla and a strong reduction of the\ncell number of thymocytes and stromal cells residing in the medulla. However, within the\nremaining medullary area, changes in cell distribution and antigen density on these cells\nwere not observed. Irradiation on the other hand causes a strong depletion of thymocytes.\nThe thymocyte population is recovered within 2 weeks and a cortical and medullary region\ncan be distinguished. CsA treatment in combination with irradiation results in a strongly\ninhibited recovery of the medulla during CsA treatment, whereas the cortex recovers to\nnormal size and morphology. The presence of the medullary IDC and epithelial cells is\nreduced proportionally to the small size of the medulla. However, the distribution of these\nstromal cells is normal. During the CsA administration, the thymuses from irradiated and\nCsA\u2010treated rats are very similar to thymuses from CsA\u2010treated rats. In conclusion, no\nchanges specific for irradiation plus CsA treatment have been observed. Regarding the\ndistribution and size of medullary stromal cells and residing thymocytes, thymuses from\nirradiated and CsA\u2010treated rats hardly differ from the thymuses from rats treated only with\nCsA. Therefore, irradiation seems essential in the generation of CsA\u2010AI by eliminating\nsuppressor\u2010cell circuits in the periphery.<\/jats:p>","DOI":"10.1155\/1995\/18495","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T07:53:31Z","timestamp":1213170811000},"page":"127-138","source":"Crossref","is-referenced-by-count":17,"title":["Differential Effects of X\u2010Irradiation and Cyclosporin\u2010AAdministration on the Thymus with Respect to theGeneration of Cyclosporin\u2010A\u2010Induced Autoimmunity"],"prefix":"10.1155","volume":"4","author":[{"given":"Leo J.J.","family":"Beijleveld","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan G.M.C.","family":"Damoiseaux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.C.","family":"Van Breda Vriesman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1994,6]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/1995\/018495.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/1995\/18495","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T21:35:29Z","timestamp":1723066529000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/1995\/18495"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,6]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,1]]}},"alternative-id":["10.1155\/1995\/18495"],"URL":"https:\/\/doi.org\/10.1155\/1995\/18495","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,6]]}}}