{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T14:10:06Z","timestamp":1723126206039},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":1826,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:p>The c\u2010<jats:italic>myc<\/jats:italic> proto\u2010oncogene linked to the mouse Thy\u20101 gene transcriptional unit predisposes\nmice to development of thymic tumors consisting predominantly of immature CD4<jats:sup>+<\/jats:sup> CD8<jats:sup>+<\/jats:sup>\ncells. In an attempt to immortalize immature T cells expressing a known T\u2010cell antigen\nreceptor (TCR), Thy\u20101\/c\u2010<jats:italic>myc<\/jats:italic> transgenic mice were bred to \u03b1\u03b2 TCR transgenic mice (F5),\nand CD4<jats:sup>+<\/jats:sup> CD8<jats:sup>+<\/jats:sup> cell lines were established from thymic tumors in double\u2010transgenic mice.\nThese cells expressed high\u2010level heat\u2010stable antigen (HSA) and were able to undergo\nprogrammed cell death upon induction with steroids and CD3 cross\u2010linking, but not with\ncognate peptide. In addition, one line had rearranged and transcribed endogenous TCR c\nand genes, in spite of the fact that transgenic \u03b1 and \u03b2 genes were also expressed.\nFurthermore, we show that Thy\u20101\/<jats:italic>myc<\/jats:italic> transgenic mice deficient in recombination activating\ngene\u20101 (RAG\u20101) do not develop tumors, in contrast to RAG\u20101<jats:sup>-<\/jats:sup>\/<jats:sup>-<\/jats:sup> mice, which are also\ntransgenic for both Thy\u20101\/myc and the F5 TCR. This indicates that in order for thymocytes\nto be transformed by the Thy\u2010myc transgene, they need to proceed to the double\u2010positive\nstage.<\/jats:p>","DOI":"10.1155\/1995\/93271","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T07:53:31Z","timestamp":1213170811000},"page":"279-288","source":"Crossref","is-referenced-by-count":1,"title":["The Programmed Cell Death of an Immature ThymocyteCell Line Transgenic for an \u03b1\u03b2 TCR and the c\u2010<i>myc<\/i>Proto\u2010Oncogene"],"prefix":"10.1155","volume":"4","author":[{"given":"Marianna","family":"Murdjeva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujiro","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trisha","family":"Norton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitris","family":"Kioussis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1995,5,23]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/1995\/093271.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/1995\/093271.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/1995\/93271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T13:39:25Z","timestamp":1723124365000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/1995\/93271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1995,1]]}},"alternative-id":["10.1155\/1995\/93271"],"URL":"https:\/\/doi.org\/10.1155\/1995\/93271","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"type":"print","value":"2314-8861"},{"type":"electronic","value":"2314-7156"}],"subject":[],"published":{"date-parts":[[1995,1]]}}}