{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T23:22:55Z","timestamp":1770765775779,"version":"3.50.0"},"reference-count":0,"publisher":"Wiley","issue":"3-4","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":865,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:p>Presented here is a cell\u2010suspension model for positive selection using thymocytes from <jats:italic>\u03b1\u03b2<\/jats:italic>\u2010TCR\n(H\u20102D<jats:sup>b<\/jats:sup>\u2010restricted) transgenic mice specific to the lymphocytic choriomeningitis virus (LCMV)\non a nonselecting MHC background (H\u20102<jats:sup>d<\/jats:sup> or TAP\u20101 \u2013\/\u2013), cocultured with freshly isolated adult\nthymus stromal cells of the selecting MHC type. The thymic stromal cells alone induced positive\nselection of functional CD4<jats:sup>-<\/jats:sup>CD8<jats:sup>+<\/jats:sup> cells whose kinetics and efficiency were enhanced by\nnominal peptide. Fibroblasts expressing the selecting MHC alone did not induce positive\nselection; however, together with nonselecting stroma and nominal peptide, there was inefficient\npositive. These results suggest multiple signaling in positive selection with selection events able\nto occur on multiple\u2010cell types. The ease with which this model can be manipulated should\ngreatly facilitate the resolution of the mechanisms of positive selection in normal and\npathological states.<\/jats:p>","DOI":"10.1155\/1998\/10534","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T07:58:53Z","timestamp":1213171133000},"page":"157-170","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["An Adult Thymic Stromal\u2010Cell Suspension Model for <i>in Vitro<\/i> Positive Selection"],"prefix":"10.1155","volume":"6","author":[{"given":"Ann P.","family":"Chidgey","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanspeter","family":"Pircher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Robson","family":"Macdonald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard L.","family":"Boyd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1997,8,19]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/1998\/010534.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/1998\/10534","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T11:39:32Z","timestamp":1723117172000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/1998\/10534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,8,19]]},"references-count":0,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[1998,1]]}},"alternative-id":["10.1155\/1998\/10534"],"URL":"https:\/\/doi.org\/10.1155\/1998\/10534","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,8,19]]},"assertion":[{"value":"1997-01-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-08-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-08-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}