{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T12:40:08Z","timestamp":1723120808928},"reference-count":0,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":967,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:p>The mouse IgG1 monoclonal antibody (mAb) K21 recognizes a 230\u2010kD molecule (K21\u2010Ag) on Hassall\u2032s corpuscles in the human thymus. This mAb also stains cultured thymic epithelial cells as well as other epithelial cell lines, revealing a predominant intracellular localization. Further analysis with mAb K21 on other lymphoid tissues showed that it also stains cells within the germinal centers of human tonsils, both lymphoid (B) cells and some with the appearance of follicular dendritic cells. Double immunostaining of tonsil sections shows that K21\u2010Ag is not expressed by T cells, whereas staining with anti\u2010CD22 and \u2010CD23 mAb revealed some doublepositive cells. A subpopulation of the lymphoid cells express the K21\u2010Ag much more strongly. This K21<jats:sup>++<\/jats:sup>\/CD23<jats:sup>++<\/jats:sup> subpopulation of cells is localized in the apical light zone of germinal centers, suggesting that K21\u2010Ag may be an important marker for the selected centrocytes within germinal centers and may play a role in B\u2010cell selection and\/or development of B\u2010cell memory. Flow cytometric analysis showed that K21\u2010Ag is expressed on the surface of a very low percentage of thymocytes, tonsillar lymphocytes, and peripheral blood mononuclear cells. Analysis of purified\/separated tonsillar T and B lymphocytes showed that T cells do not express the K21\u2010Ag; in contrast, B cells express low levels of the K21\u2010Ag, and this together with CD23 is upregulated after mitogenic stimulation. Our data therefore raise the possibility that the K2l\u2010 Ag may play a role in B\u2010lymphocyte activation\/selection.<\/jats:p>","DOI":"10.1155\/1998\/29340","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T07:58:53Z","timestamp":1213171133000},"page":"41-52","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["K21\u2010Antigen: A Molecule Shared by the Microenvironments of the Human Thymus and Germinal Centers"],"prefix":"10.1155","volume":"6","author":[{"given":"Nesrina","family":"Imami","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather M.","family":"Ladyman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bjarne","family":"Vincents","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulhamid","family":"Al-Tubuly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jona","family":"Freysd\u00f3ttir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moditi L.","family":"Sedibane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Taylor-Fishwick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian M.J.","family":"Foxwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary A.","family":"Ritter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1997,5,9]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/1998\/029340.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/1998\/29340","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T11:31:53Z","timestamp":1723116713000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/1998\/29340"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,5,9]]},"references-count":0,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[1998,1]]}},"alternative-id":["10.1155\/1998\/29340"],"URL":"https:\/\/doi.org\/10.1155\/1998\/29340","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"type":"print","value":"2314-8861"},{"type":"electronic","value":"2314-7156"}],"subject":[],"published":{"date-parts":[[1997,5,9]]},"assertion":[{"value":"1996-10-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-05-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-05-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}