{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T07:10:30Z","timestamp":1722323430613},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":730,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Cellular Pathology"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:p>Two\u2010color, multiparametric synthesis phase fraction (SPF) analysis of cytokeratin\u2010labeled epithelial cells was flow cytometrically performed on both benign (SPFb) and malignant tissue samples (if available, SPFt) from 132 mastectomy\/lumpectomy specimens. These data were then correlated with clinicopathologic features, including (1) tumor differentiation, (2) the proportion of tumor comprised of duct carcinoma\u2010<jats:italic>in situ<\/jats:italic> (DCIS), and (3) the histology of accompanying benign breast tissue, classified by predominant microscopic pattern as intact, normal terminal duct lobular units (NTDLU, 34% of cases), atrophic (AT, 33% of cases), proliferative fibrocystic (PFC, 26% of cases), and non\u2010proliferative fibrocystic (NPFC, 7% of cases). SPFt was inversely correlated with extent of DCIS (DCIS =0 \u2013 20% tumor volume \u2013 12.7% mean SPFt, vs. DCIS &gt;20% tumor volume \u2013 6.4% mean SPFt, <jats:italic>p<\/jats:italic> = 0.001). SPFt also correlated with the histology of background benign breast tissue (NTDLU \u2013 14.8% mean SPFt vs. AT \u2013 6.9% mean SPFt vs. PFC \u2013 12.7% mean SPFt, <jats:italic>p<\/jats:italic> = 0.05) but it did not correlate with patient age or SPFb (overall mean =0.73%). SPFb was correlated with patient age (&gt;56 yr \u2013 0.59% mean SPFb vs. &lt; yr \u2013 0.84% mean SPFb, <jats:italic>p<\/jats:italic> = 0.02), with background histology (NTDLU \u2013 1.1% mean SPFb vs. AT \u2013 0.43% mean SPFb vs. PFC \u2013 0.70% mean SPFb, p &lt; 0.02) and with the grade of the neoplasm (well\/moderate \u2013 0.58% mean vs. poorly differentiated \u2013 0.85% mean, <jats:italic>p<\/jats:italic> = 0.04). Patients having a background of PFC were significantly older than patients with a background of NTDLU (45.2 yr vs. 60.2 yr, <jats:italic>p<\/jats:italic> = 0.01).<\/jats:p><jats:p>We conclude: (1) breast carcinomas arising from a background of more actively cycling pre\u2010involutional or proliferative fibrocystic epithelium have a greater proliferative fraction than tumors arising from atrophic epithelium, implying that the differentiation status of target cells may impact the effect(s) of tumorigenic events; (2) PFC may represent delayed, abnormal or interrupted involution rather than a hyperproliferative state relative to NTDLU, suggesting that it facilitates neoplasia by extending the period of exposure to promoter agents such as endogenous hormones, and (3) lower SPFt in breast neoplasia with more abundant \u201cresidual\u201d DCIS may reflect a lengthier pre\u2010invasive disease interval due to intrinsically less aggressive phenotype.<\/jats:p>","DOI":"10.1155\/1998\/294903","type":"journal-article","created":{"date-parts":[[2015,1,12]],"date-time":"2015-01-12T14:27:49Z","timestamp":1421072869000},"page":"167-175","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Histopathologic and Flow\u2010Cytometric Analysis of Neoplastic and Benign \u201cbackground\u201d Tissue in Breast Carcinoma Resections"],"prefix":"10.1155","volume":"17","author":[{"given":"Daniel W.","family":"Visscher","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debra S.","family":"Gingrich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Leon-Armin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"Tabaczka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D.","family":"Crissman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1998,8,27]]},"container-title":["Analytical Cellular Pathology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/acp\/1998\/294903.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/1998\/294903","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T06:44:01Z","timestamp":1722321841000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/1998\/294903"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1998,1]]}},"alternative-id":["10.1155\/1998\/294903"],"URL":"https:\/\/doi.org\/10.1155\/1998\/294903","archive":["Portico"],"relation":{},"ISSN":["2210-7177","2210-7185"],"issn-type":[{"type":"print","value":"2210-7177"},{"type":"electronic","value":"2210-7185"}],"subject":[],"published":{"date-parts":[[1998,1]]},"assertion":[{"value":"1998-06-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-08-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-08-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}