{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T06:46:18Z","timestamp":1777531578179,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":730,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100000275","name":"Leverhulme Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000275","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001314","name":"Durham University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001314","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:p>A novel apoptosis\u2010specific protein (ASP) has recently been identified in the cytoplasm of\napoptotic mammalian cells. This paper investigates whether ASP is found in <jats:italic>Xenopus<\/jats:italic> thymus\ntumor\u2010derived lymphoid cell lines undergoing apoptosis and also in apoptotic, nontransformed\nsplenocytes. Cultured <jats:italic>Xenopus<\/jats:italic> tumor lymphoid cells induced to undergo, apoptosis by serum\ndeprivation or treatment with the calcium ionophore, ionomycin, displayed altered morphology\ntypical of apoptotic cells, as judged by flow cytometric light\u2010scatter characteristics and by\nfluorescence microscopy of acridine\u2010orange\u2010stained cells. Flow cytometry of permeabilized cells\nand fluorescence microscopy of acetone\u2010fixed cytospins revealed that apoptotic <jats:italic>Xenopus<\/jats:italic> tumor\ncells, especially those displaying loss or condensation of DNA, displayed increased expression\nof epitopes recognized by a rabbit polyclonal antibody against ASP. Flow cytometry confirmed\nthat ASP is also expressed in splenocytes induced to apoptose by culture in ionomycin or\nfollowing concanavalin A stimulation. No increased expression of ASP was seen when lymphoid\ntumor cells or splenocytes were induced into necrosis by overdose with the antifungal agent\namphotericin B. Western blotting with antibody against ASP identified the emergence of several\nprotein bands in cell lysates from apoptotic, but not necrotic, <jats:italic>Xenopus<\/jats:italic> tumor cells. The new and\nsimple methodology for identifying apoptotic cells described here is likely to be of value to those\nstudying immune system development and associated programmed cell death in <jats:italic>Xenopus<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/1998\/70616","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T07:54:53Z","timestamp":1213170893000},"page":"333-348","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Apoptosis\u2010Specific Protein (ASP) Identified in Apoptotic <i>Xenopus<\/i> Thymus Tumor Cells"],"prefix":"10.1155","volume":"5","author":[{"given":"John","family":"Horton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Milner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trudy","family":"Horton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"Ritchie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duncan","family":"Gascoyne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Hewson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ester","family":"Hammond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Gregory","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Grand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1998,1,5]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/1998\/070616.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/1998\/70616","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T11:31:42Z","timestamp":1723116702000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/1998\/70616"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1998,1]]}},"alternative-id":["10.1155\/1998\/70616"],"URL":"https:\/\/doi.org\/10.1155\/1998\/70616","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,1]]},"assertion":[{"value":"1997-08-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-01-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-01-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}