{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T16:32:09Z","timestamp":1770481929558,"version":"3.49.0"},"reference-count":0,"publisher":"Wiley","issue":"2-4","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministero dell'Universit\u00e0 e della Ricerca Scientifica e Tecnologica"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:p>Matrix metalloproteinase (MMP) expression and production are associated with\nadvanced\u2010stage tumor and contribute to tumor progression, invasion and metastases. The current\nstudy was designed to determine the expression and production of MMP\u20102 (gelatinase A)\nand MMP\u20109 (gelatinase B) by human lymphoid tumor cells. Changes in expression and production\nwere also investigated during tumor progression of multiple myeloma and mycosis\nfungoides. <jats:italic>In situ<\/jats:italic> hybridization analysis revealed that lymphoblastic leukemia B cells (SB\ncell line), multiple myeloma (MM) cells (U266 cell line) and lymphoblastic leukemia T cells\n(CEM and Jurkat cell lines) express constitutively the mRNA for MMP\u20102 and\/or MMP\u20109. We\ndemonstrated by gelatin\u2010zymography of cell culture medium that both enzymes were secreted\nin their cleaved (activated) form. <jats:italic>In situ<\/jats:italic> hybridization of bone marrow plasma cells and gelatin\u2010\nzymography of the medium showed that patients with active MM (diagnosis, relapse,\nleukemic progression) express higher levels of MMP\u20102 mRNA and protein than patients with\nnon\u2010active MM (complete\/objective response, plateau) and with monoclonal gammopathies\nof undetermined significance (MGUS). MMP\u20109 expression and secretion was similar in all\npatient groups. In patients with mycosis fungoides (MF), the expression of MMP\u20102 and\nMMP\u20109 mRNAs was significantly upregulated with advancing stage, in terms of lesions both\npositive for one of two mRNAs and with the greatest intensity of expression. Besides MF\ncells, the MMP\u20102 and\/or MMP\u20109 mRNAs were expressed by some stromal cell populations\n(microvascular endothelial cells, fibroblasts, macrophages), suggesting that these cells cooperate\nin the process of tumor invasion. Our studies identify MMPs as an important class of\nproteinases involved in the extracellular matrix (ECM) degradation by human lymphoid\ntumors, and suggest that MMPs inhibitors may lead to important new treatment for their control.<\/jats:p>","DOI":"10.1155\/2000\/74372","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T08:03:14Z","timestamp":1213171394000},"page":"77-88","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Proteolytic Activity of Human Lymphoid Tumor Cells.Correlation with Tumor Progression"],"prefix":"10.1155","volume":"7","author":[{"given":"Angelo","family":"Vacca","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Domenico","family":"Ribatti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Ria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Pellegrino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Bruno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Merchionne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franco","family":"Dammacco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,1]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2000\/074372.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2000\/74372","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T11:30:22Z","timestamp":1723116622000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2000\/74372"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1]]},"references-count":0,"journal-issue":{"issue":"2-4","published-print":{"date-parts":[[2000,1]]}},"alternative-id":["10.1155\/2000\/74372"],"URL":"https:\/\/doi.org\/10.1155\/2000\/74372","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,1]]},"assertion":[{"value":"2000-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}