{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:00:22Z","timestamp":1761058822331},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:p>Intrathymic injection of the Abelson murine leukemia virus (A\u2010MuLV) results in transformation\nof immature T and B lymphoid cells. In this report we demonstrate that the concentration\nof A\u2010MuLV injected into murine thymi influences the selection of the transformation target.\nThus, concentrated A\u2010MuLV gives rise to Thy\u20101<jats:sup>+<\/jats:sup> B220<jats:sup>-<\/jats:sup> thymomas. In contrast, dilute virus\ninduces B220<jats:sup>+<\/jats:sup> thymomas that also express low levels of Thy\u2010 1 (Thy\u20101<jats:sup>lo<\/jats:sup>), a phenotype that is\nsimilar to marrow\u2010derived progenitor B\u2010lymphoid cells (pro\u2010B cells) that are highly susceptible\nto A\u2010MuLV transformation <jats:italic>in<\/jats:italic><jats:italic>vitro<\/jats:italic>. However, rare B220<jats:sup>+<\/jats:sup> lymphoid cells isolated from\nnormal adult thymi were not transformed by A\u2010MuLV <jats:italic>in<\/jats:italic><jats:italic>vitro<\/jats:italic>, while B220<jats:sup>+<\/jats:sup> cells isolated\nfrom bone marrow were highly susceptible to transformation by A\u2010MuLV. The Thy\u20101<jats:sup>lo<\/jats:sup>\nB220<jats:sup>+<\/jats:sup>population in the primary thymomas had not rearranged TCR\u03b3, TCR\u03b2, or <jats:italic>I<\/jats:italic><jats:italic>g<\/jats:italic>\u03ba genes, but\ncontained subpopulations that assembled <jats:italic>Ig<\/jats:italic> DJ<jats:sub>H<\/jats:sub> or VDJ<jats:sub>H<\/jats:sub> genes and were therefore similar to\ntransformed pro\u2010 and pre\u2010B cells obtained from A\u2010MuLV infected fetal liver and adult bone\nmarrow, respectively. However, unlike A\u2010MuLV\u2010transformed pro\u2010 and pre\u2010B cells, many (40\u2013\n70%) of the Thy\u20101<jats:sup>lo<\/jats:sup> B220<jats:sup>+<\/jats:sup> transformed thymoma cells had not rearranged <jats:italic>Igh<\/jats:italic> genes, and\ntherefore appear to represent undifferentiated lymphoid cells. We conclude that A\u2010MuLV may\ntransform an undifferentiated lymphoid target in the thymus.<\/jats:p>","DOI":"10.1155\/2000\/94158","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T08:03:45Z","timestamp":1213171425000},"page":"1-18","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Transformation of Undifferentiated Thy\u20101<sup>lo<\/sup> B220<sup>+<\/sup> Thymic Lymphoid Cells by the Abelson Murine Leukemia Virus"],"prefix":"10.1155","volume":"8","author":[{"given":"Eric Y.","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradley J.","family":"Swanson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven S.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,1]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2000\/094158.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2000\/094158.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2000\/94158","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T11:55:19Z","timestamp":1723118119000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2000\/94158"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,1]]}},"alternative-id":["10.1155\/2000\/94158"],"URL":"https:\/\/doi.org\/10.1155\/2000\/94158","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"type":"print","value":"2314-8861"},{"type":"electronic","value":"2314-7156"}],"subject":[],"published":{"date-parts":[[2000,1]]},"assertion":[{"value":"2000-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}