{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T05:55:58Z","timestamp":1760853358497},"reference-count":0,"publisher":"Wiley","issue":"3-4","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:p>Prion diseases are fatal neurodegenerative disorders caused by accumulation of abnormal\nprion protein (protease\u2010resistant prion, PrPres). PrPres accumulation is also detected in lymphoid\norgans after peripheral infection. Several studies suggest that follicular dendritic cells\n(FDC) could be the site of PrPres retention and amplification.<\/jats:p><jats:p>Here we show that human follicular dendritic cells can express normal cellular prion protein\n(PrPc) both <jats:italic>in<\/jats:italic><jats:italic>situ<\/jats:italic> and <jats:italic>in<\/jats:italic><jats:italic>vitro<\/jats:italic>. When tonsillar cryosections were treated with anti\u2010PrP\nantibody, the label was found on some very delicate cell extensions inside the lymphoid follicles,\nespecially in the germinal centres. These extensions react with DRC1 antibody, used frequently\nto label FDC. Other structures labelled with anti\u2010PrP antibody were the keratinocytes.<\/jats:p><jats:p>To confirm the ability of FDC to synthesise PrPc, we isolated FDC by a non\u2010enzymatic\nprocedure and cultured them. By cytochemistry and flow cytometry it was clearly shown that\nFDC do produce PrPc.<\/jats:p>","DOI":"10.1155\/2001\/45454","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T08:03:45Z","timestamp":1213171425000},"page":"259-266","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Human FDC express PrPc <i>in<\/i><i>vivo<\/i> and <i>in<\/i><i>vitro<\/i>"],"prefix":"10.1155","volume":"8","author":[{"given":"Caroline","family":"Thielen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadine","family":"Antoine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"France","family":"M\u00e9lot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Yves","family":"Cesbron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernst","family":"Heinen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rikiya","family":"Tsunoda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,1]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2001\/045454.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2001\/45454","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T11:36:14Z","timestamp":1723116974000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2001\/45454"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1]]},"references-count":0,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2001,1]]}},"alternative-id":["10.1155\/2001\/45454"],"URL":"https:\/\/doi.org\/10.1155\/2001\/45454","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"type":"print","value":"2314-8861"},{"type":"electronic","value":"2314-7156"}],"subject":[],"published":{"date-parts":[[2001,1]]},"assertion":[{"value":"2001-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}