{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T12:40:06Z","timestamp":1723120806252},"reference-count":0,"publisher":"Wiley","issue":"3-4","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:p>The major histocompatibility complex (MHC) class II is involved both in thymocyte maturation\nand peptide presentation and might thus play a key role in the pathogenesis of paraneoplastic\nmyasthenia gravis (MG) in thymomas. To further investigate this issue, we analyzed\nand scored the expression of epithelial class II expression in 35 thymomas (medullary, MDT;\nmixed, MXT; cortical and well differentiated thymic carcinoma, CT \/ WDTC) and correlated\nit with the histological tumor subtype, prevalence of MG and thymocyte maturation, which\nwas analyzed by flow cytometry and RT\u2010PCR. Our results show that both MHC class II\nexpression and thymocyte maturation are highly dependent on the histological tumor subtype.\nCT \/ WDTC retain features of the normal outer thymic cortex, namely substantial MHC class\nII expression together with normal early thymocyte maturation until late phases of positive\nselection, but disturbed terminal thymopoiesis. By contrast, MDT and MXT retain features of\nthe normal inner cortex and the medulla with low to absent class II expression and highly\nabnormal early thymocyte maturation including impaired positive selection, while terminal T\ncell maturation in MXT appeared undisturbed. There was no correlation between MHC class\nII expression and MG status for a given tumor subtype. In conclusion, our results provide evidence\nfor a different histogenesis of cortical thymomas and well differentiated carcinomas on\nthe one hand and mixed and medullary thymomas on the other.<\/jats:p><jats:p>Decreased expression levels of MHC class II, although of crucial importance for abnormal\nintratumorous maturation, are not sufficient to explain the emergence of paraneoplastic MG.<\/jats:p>","DOI":"10.1155\/2001\/49127","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T08:03:45Z","timestamp":1213171425000},"page":"279-290","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Evidence for Distinct Mechanisms in the Shapingof the CD4 T Cell Repertoire in Histologically DistinctMyasthenia Gravis \u2013 Associated Thymomas"],"prefix":"10.1155","volume":"8","author":[{"given":"P.","family":"Str\u00f6bel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Helmreich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Kalbacher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. K.","family":"M\u00fcller-Hermelink","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Marx","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,1]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2001\/049127.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2001\/49127","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T11:36:22Z","timestamp":1723116982000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2001\/49127"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1]]},"references-count":0,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2001,1]]}},"alternative-id":["10.1155\/2001\/49127"],"URL":"https:\/\/doi.org\/10.1155\/2001\/49127","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"type":"print","value":"2314-8861"},{"type":"electronic","value":"2314-7156"}],"subject":[],"published":{"date-parts":[[2001,1]]},"assertion":[{"value":"2001-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}