{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T21:15:42Z","timestamp":1774473342875,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"NATO Fellowship"},{"name":"Ida MacLean Fellowship"},{"name":"Helga Jonsdottir and Sigvaldi Kristjansson Memorial Fund"},{"name":"Memorial Fund of Bergthora Magnusdottir and Jakob Bjarnason"},{"name":"Theodor Johnson\u2019s Memorial Fund"},{"name":"Islandsbanki\u2019s Studentline"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:p>Monoclonal antibody (mAb) MR6 recognises a 200 kDa glycoprotein, gp200\u2010MR6, which is\nexpressed at high levels on the surface of human thymic cortical epithelium. In order to produce\nfurther mAbs against the gp200\u2010MR6 molecule, mice were immunised with purified\nhuman gp200\u2010MR6, hybridomas produced and supernatants screened for MR6\u2010like reactivity\non human thymic sections. Surprisingly this conventional hybridoma technique failed to produce\nstable hybridoma cells producing MR6\u2010like antibodies. However, antibodies with specificitie\nother than MR6\u2010like were obtained. Three such antibodies (1B2, 3A3 and 4B3) were\nanalysed further. Expression of 1B2\u2010antigen, 3A3\u2010antigen and 4B3\u2010antigen was analysed on\nskin, tonsil and thymic sections, on cultured thymic epithelial cells (TEC), thymocytes and\nperipheral blood mononuclear cells (PBMC), and found to be expressed by both lymphocytes\nand epithelial cell populations. Furthermore, the antigens were also expressed on mouse\nthymic, epithelial cells. The regulation of expression of these antigens was analysed following\nmitogen or cytokine stimulation of PBMC and cultured TEC, respectively. Expression on T\ncells was clearly affected by mitogens that mimic activation through the T cell receptor and\nexpression on cultured TEC was affected by T cell\u2010derived cytokines. Thus, the shared epithelial\u2010\nlymphocyte molecules identified in this study may play a role in the cross\u2010talk\nbetween the developing thymocytes and their epithelial microenvironment. The production of\nmAbs with specificities other than that of purified gp200\u2010MR6 indicates that a wide range of\nB cells with specificity for components of the human thymic microenvironment exist in the\nnormal mouse. These may detect epitopes that are shared with common pathogens to which\nthe animals are exposed. Alternatively, they may be autoreactive B cells that are normally\nsilent in the absence of T cell help. This help may be provided by T cells specific for human\ngp200\u2010MR6, or nonspecifically by polyclonal activation induced by the adjuvant.<\/jats:p>","DOI":"10.1155\/2001\/59678","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T08:03:45Z","timestamp":1213171425000},"page":"75-93","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antibodies to Human Thymic Epithelium Form Partof the Murine Autoreactive Repertoire"],"prefix":"10.1155","volume":"8","author":[{"given":"Jona","family":"Freysdottir","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary A.","family":"Ritter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,1]]},"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2001\/059678.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2001\/59678","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T17:31:53Z","timestamp":1723138313000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2001\/59678"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,1]]}},"alternative-id":["10.1155\/2001\/59678"],"URL":"https:\/\/doi.org\/10.1155\/2001\/59678","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,1]]},"assertion":[{"value":"2001-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}