{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T11:09:00Z","timestamp":1750158540321,"version":"3.37.3"},"reference-count":0,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000019","name":"Crohn\u2019s and Colitis Foundation of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000019","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Canadian Journal of Gastroenterology and Hepatology"],"published-print":{"date-parts":[[2001,1]]},"abstract":"OBJECTIVE: To develop a serological test to measure antibodies to Saccharomyces cerevisiae<\/jats:italic> in patients with inflammatory bowel disease.<\/jats:p>METHODS: An ELISA to the mannan of S cerevisiae<\/jats:italic> that is commercially available was developed. Sera were tested from randomly chosen sera specimens kept frozen at the University of Manitoba Inflammatory Bowel Disease Clinical and Research Centre, Winnipeg, Manitoba. Clinical diagnoses were kept blinded until the assay results were finalized. One hundred thirty\u2010six sera were tested, including 51 with Crohn\u2032s disease, 32 with ulcerative colitis, one with indeterminate colitis and 16 other control subjects. Thirty\u2010six samples were duplicates from patients already studied but were either run on separate days or drawn on different days.<\/jats:p>RESULTS: Using a cutoff of 15 binding units as a positive result, Crohn\u2032s disease was found to have a sensitivity of 53% but a specificity of 100% compared with ulcerative colitis. Compared with all other diagnoses (including ulcerative colitis), Crohn\u2032s disease had a sensitivity of 53% and a specificity of 96%. For patients with Crohn\u2032s disease only, those who were anti\u2010S cerevisiae<\/jats:italic> antibody (ASCA) positive (n=27) were significantly more likely to have proximal gastrointestinal disease and significantly less likely to have colonic or inflammatory type disease than those who were ASCA negative (n=24). The direct cost of this assay was $6.00 per positive test, and the total charge was set at $38.15.<\/jats:p>CONCLUSIONS: A reasonably inexpensive, easy and reproducible assay to assess for antibodies to S cerevisiae<\/jats:italic> has been developed. Using a cutoff for positivity of 15 binding units, this test had a specificity of 100% for ruling out Crohn\u2032s disease and a lower (60%) sensitivity compared with ulcerative colitis. This test could identify a specific phenotype of patients with Crohn\u2032s disease as being more likely to have small bowel Crohn\u2032s disease and less likely to have colonic (isolated) or inflammatory disease, as opposed to fibrostenotic disease or penetrating disease. The test proved reliable when assaying samples drawn or assayed on different days.<\/jats:p>","DOI":"10.1155\/2001\/605470","type":"journal-article","created":{"date-parts":[[2016,3,24]],"date-time":"2016-03-24T21:01:53Z","timestamp":1458853313000},"page":"499-504","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Development of an Assay for Antibodies to Saccharomyces cerevisiae<\/i>: Easy, Cheap and Specific for Crohn\u2032s Disease"],"prefix":"10.1155","volume":"15","author":[{"given":"Charles N","family":"Bernstein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Orr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James F","family":"Blanchard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Sargent","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donna","family":"Workman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,8]]},"container-title":["Canadian Journal of Gastroenterology and Hepatology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/cjgh\/2001\/605470.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2001\/605470","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T08:22:49Z","timestamp":1722327769000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2001\/605470"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2001,1]]}},"alternative-id":["10.1155\/2001\/605470"],"URL":"https:\/\/doi.org\/10.1155\/2001\/605470","archive":["Portico"],"relation":{},"ISSN":["2291-2789","2291-2797"],"issn-type":[{"type":"print","value":"2291-2789"},{"type":"electronic","value":"2291-2797"}],"subject":[],"published":{"date-parts":[[2001,1]]},"assertion":[{"value":"2001-03-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-04-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-08-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}