{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T10:10:03Z","timestamp":1722334203709},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Analytical Cellular Pathology"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:p><jats:italic>Aim<\/jats:italic> To test laser scanning cytometry (LSC) for the analysis of ploidy in squamous cell carcinoma of the hypopharynx (SCCH) and to develop a routine application for minimal samples such as fine needle aspirate biopsies (FNABs). Methods: From 11 individuals 30 FNABs of primary tumors (n=11) and lymphatic metastases of SCCH (n=11) and non\u2010metastatic lymph nodes (n=8) are analyzed by LSC. This microscope based instrument scans the cells after immobilization on a glass slide and after double staining of cytokeratin and DNA. The location of each cell is stored with the fluorescence data. Therefore the morphology of every cell can be documented by re\u2010staining with H&amp;E and re\u2010localization on the slide. Additionally, aliquots are Feulgen\u2010stained for image cytometry in 8 specimens. Results: The diploid reference peak is identified taking leukocytes as internal standard. The DNA\u2010index of the carcinoma cells ranges from 0.4 to 3.8. Comparison with image cytometry shows good correlation (r=0.89). <jats:italic>Conclusion<\/jats:italic>: LSC provides a reliable and objective way to determine the ploidy of SCCH pre\u2010operatively. Colour figures can be viewed on <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.esacp.org\/acp\/2003\/25-2\/gerstner.htm\">http:\/\/www.esacp.org\/acp\/2003\/25\u20102\/gerstner.htm<\/jats:ext-link>.<\/jats:p>","DOI":"10.1155\/2003\/971748","type":"journal-article","created":{"date-parts":[[2015,1,12]],"date-time":"2015-01-12T14:09:39Z","timestamp":1421071779000},"page":"51-62","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Analysis of Ploidy in Hypopharyngeal Cancer by Laser Scanning Cytometry on Fine Needle Aspirate Biopsies"],"prefix":"10.1155","volume":"25","author":[{"given":"Andreas O.H.","family":"Gerstner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Machlitt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans\u2010J\u00fcrgen","family":"Welkoborsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friedrich","family":"Bootz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Attila","family":"T\u00e1rnok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,7,10]]},"container-title":["Analytical Cellular Pathology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/acp\/2003\/971748.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2003\/971748","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T09:55:51Z","timestamp":1722333351000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2003\/971748"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,7,10]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1155\/2003\/971748"],"URL":"https:\/\/doi.org\/10.1155\/2003\/971748","archive":["Portico"],"relation":{},"ISSN":["2210-7177","2210-7185"],"issn-type":[{"type":"print","value":"2210-7177"},{"type":"electronic","value":"2210-7185"}],"subject":[],"published":{"date-parts":[[2002,7,10]]},"assertion":[{"value":"2002-02-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-07-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-07-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}