{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T14:10:09Z","timestamp":1722348609325},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Canadian Respiratory Journal"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:p><jats:bold>BACKGROUND:<\/jats:bold> Interleukin (IL)\u201010 is a pleiotropic cytokine released from various cells, including T cells. Although IL\u201010 is suggested to inhibit allergic responses, its role in asthma remains uncertain. The purpose of the present study was to compare the profile of IL\u201010 in circulating T cells from stable atopic asthmatics, atopic nonasthmatics and healthy controls.<\/jats:p><jats:p><jats:bold>METHODS:<\/jats:bold> Peripheral blood mononuclear cells were isolated, stained with anti\u2010CD3 and CD4\/CD8 antibodies, and then processed for intracellular IL\u201010 detection by flow cytometry.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> A kinetic study in healthy controls showed that stimulation with phorbol 12\u2010myristate 13\u2010acetate and ionomycin significantly increased the frequencies of IL\u201010\u2010producing CD3<jats:sup>+<\/jats:sup>, CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> cells. Without stimulation, the frequencies of IL\u201010\u2010producing CD3<jats:sup>+<\/jats:sup>, CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup>cells were significantly higher in asthmatics than in healthy controls, while a similar trend was observed in atopic nonasthmatics. Stimulation for 24 h significantly increased IL\u201010\u2010producing CD3<jats:sup>+<\/jats:sup>, CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup>cells in healthy controls and atopic nonasthmatics, but not in asthmatics.<\/jats:p><jats:p><jats:bold>CONCLUSIONS:<\/jats:bold> The frequency of IL\u201010\u2010producing T cells is increased in the circulation of stable atopic asthmatics compared with normal controls. The lack of enhancement in their frequency by phorbol 12\u2010myristate 13\u2010acetate and ionomycin in asthmatics suggests that the circulating T cells of asthmatic subjects are maximally stimulated with regards to IL\u201010 production; alternatively, IL\u201010 production by T cells from asthmatics may be regulated differently than T cells from other subjects.<\/jats:p>","DOI":"10.1155\/2004\/710962","type":"journal-article","created":{"date-parts":[[2016,3,15]],"date-time":"2016-03-15T08:03:11Z","timestamp":1458028991000},"page":"33-38","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Different Profile of Interleukin\u201010 Production in Circulating T Cells from Atopic Asthmatics Compared with Healthy Subjects"],"prefix":"10.1155","volume":"11","author":[{"given":"K","family":"Matsumoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Narita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Rerecich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DP","family":"Snider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PM","family":"O\u2032Byrne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,3]]},"container-title":["Canadian Respiratory Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/crj\/2004\/710962.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2004\/710962","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T13:34:57Z","timestamp":1722346497000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2004\/710962"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1155\/2004\/710962"],"URL":"https:\/\/doi.org\/10.1155\/2004\/710962","archive":["Portico"],"relation":{},"ISSN":["1198-2241","1916-7245"],"issn-type":[{"type":"print","value":"1198-2241"},{"type":"electronic","value":"1916-7245"}],"subject":[],"published":{"date-parts":[[2004,1]]},"assertion":[{"value":"2004-02-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}