{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T22:32:39Z","timestamp":1791757959011,"version":"4.3.4"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2007]]},"abstract":"<jats:p><jats:italic>Aims<\/jats:italic>. We investigated the effect of HR-HPV infection on the capacity of the cytokine network in whole blood cultures during carcinogenesis of cervical carcinoma.<jats:italic>Methods<\/jats:italic>. Thirty-nine women with moderate dysplasia, severe dysplasia, cervical carcinoma, or without dysplasia formed the study group. The control group consisted of 10 HR-HPV-negative women without CIN. Whole blood cultures were stimulated with phytohemagglutinin (PHA) and concentrations of tumour necrosis factor<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"E2\"><mml:mi>\u03b1<\/mml:mi><\/mml:math>(TNF<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"E3\"><mml:mi>\u03b1<\/mml:mi><\/mml:math>), interferon<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"E4\"><mml:mi>\u03b3<\/mml:mi><\/mml:math>(IFN<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"E5\"><mml:mi>\u03b3<\/mml:mi><\/mml:math>), interleukin 2 (IL-2), interleukin 12 (IL-12), interleukin 4 (IL-4), and interleukin 10 (IL-10) were determined by ELISAs.<jats:italic>Results<\/jats:italic>. A significant increase in cytokine release was detected in HR-HPV-positive women without dysplasia. In women with cervical cancer, release of IFN<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"E6\"><mml:mi>\u03b3<\/mml:mi><\/mml:math>and IL-12 was of the same magnitude as in HR-HPV-positive women without clinical manifestations. Most Th1-type\/Th2-type ratios decreased form CIN II to CIN III, and increased from CIN III to invasive carcinoma.<jats:italic>Conclusions<\/jats:italic>. (1) Infection with HR-HPV without expression of cervical dysplasia induces activation of the cytokine network. (2) Increases in ratios of Th1-type to Th2-type cytokines at the stage of cervical carcinoma were found by comparison with stage CIN III. (3) Significant changes in the kinetics of cytokine release to a Th2-type immune response in blood of women with cervical dysplasia occurred progressively from CIN II to CIN III.<\/jats:p>","DOI":"10.1155\/2007\/24147","type":"journal-article","created":{"date-parts":[[2007,12,13]],"date-time":"2007-12-13T18:41:26Z","timestamp":1197571286000},"page":"1-8","source":"Crossref","is-referenced-by-count":28,"title":["Cytokine Release in HR-HPV(+) Women without and with Cervical Dysplasia (CIN II and III) or Carcinoma, Compared with HR-HPV(<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"E1\"><mml:mrow><mml:mstyle mathvariant=\"bold\"><mml:mo>\u2212<\/mml:mo><\/mml:mstyle><\/mml:mrow><\/mml:math>) Controls"],"prefix":"10.1155","volume":"2007","author":[{"given":"Aagje G.","family":"Bais","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilse","family":"Beckmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia C.","family":"Ewing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marinus J. C.","family":"Eijkemans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris J. L. M.","family":"Meijer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J. F.","family":"Snijders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo J. 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