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Further, we have conjugated these gold nanoparticles\n    to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation\n    protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of\n    the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the\n    characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular\n    probes in the field of biomedical imaging are also discussed.<\/jats:p>","DOI":"10.1155\/2007\/29817","type":"journal-article","created":{"date-parts":[[2007,9,5]],"date-time":"2007-09-05T08:07:45Z","timestamp":1188979665000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":108,"title":["Synthesis and Bioconjugation of Gold Nanoparticles as Potential Molecular Probes for Light\u2010Based Imaging Techniques"],"prefix":"10.1155","volume":"2007","author":[{"given":"Raja Gopal","family":"Rayavarapu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilma","family":"Petersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantin","family":"Ungureanu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janine N.","family":"Post","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ton G.","family":"van Leeuwen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srirang","family":"Manohar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,8,19]]},"reference":[{"key":"e_1_2_1_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth815"},{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth818"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt892"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1109\/79.962278"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.2195024"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physchem.47.1.555"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.neo.7900082"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-46009-8_1"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1118\/1.1573791"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.2141825"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2005.01.030"},{"key":"e_1_2_1_12_2","first-page":"1999","article-title":"Real-time vital optical imaging of precancer using anti-epidermal growth factor\nreceptor antibodies conjugated to gold nanoparticles","volume":"63","author":"Sokolov K.","year":"2003","journal-title":"Cancer Research"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1049\/ip-nbt:20045012"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/cm0492336"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp0516846"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4095(200109)13:18<1389::AID-ADMA1389>3.0.CO;2-F"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/cm020732l"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/la049463z"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp0570972"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3798106"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-333927-0.50007-6"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/la010530o"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp054808n"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012342335-1\/50015-4"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja057254a"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mibio.2004.06.002"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","unstructured":"EghtedariM. 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