{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T02:13:20Z","timestamp":1776824000866,"version":"3.51.2"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,6,23]],"date-time":"2008-06-23T00:00:00Z","timestamp":1214179200000},"content-version":"vor","delay-in-days":174,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Polish Research Committee","award":["3 PO5B 12225"],"award-info":[{"award-number":["3 PO5B 12225"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Cell signaling via Toll\u2010like receptors (TLRs) leads to synovial inflammation in rheumatoid arthritis (RA). We aimed to assess effects of TLR2 and TLR4 stimulation on proinflammatory cytokine production by peripheral blood mononuclear cells (PBMCs) from patients with recent\u2010onset RA, osteoarthrosis (OA), and healthy control (HC).\n<jats:italic>Methods<\/jats:italic>. PBMCs were stimulated with LPS, biglycan and cytokine mix. Cytokines were analyzed in supernatants with ELISA. Expression of toll\u2010like receptors mRNA in leukocytes was analyzed using real\u2010time qPCR.\n<jats:italic>Results<\/jats:italic>. PBMCs from RA patients spontaneously produced less IL\u20106 and TNF<jats:italic>\u03b1<\/jats:italic> than cells from OA and HC subjects. \nLPS increased cytokines\u2032 production in all groups. In RA patients increase was dramatic (30 to 48\u2010fold and 17 to 31\u2010fold, for respective cytokines) compared to moderate (2 to 8\u2010fold) in other groups. LPS induced 15\u2010HETE generation in PBMCs from RA (mean 251%) and OA patients (mean 43%), although  only in OA group, the increase was significant. TLR2 and TLR4 gene expressions decreased in response to cytokine mix, while LPS enhanced TLR2 expression in HC and depressed TLR4 expression in OA patients.\n<jats:italic>Conclusion<\/jats:italic>. PBMCs from recent\u2010onset RA patients are overresponsive to stimulation with bacterial lipopolysaccharide. \nTLR expression is differentially regulated in healthy and arthritic subjects.<\/jats:p>","DOI":"10.1155\/2008\/132732","type":"journal-article","created":{"date-parts":[[2008,6,24]],"date-time":"2008-06-24T10:45:59Z","timestamp":1214304359000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Increased Responsiveness to Toll\u2010Like Receptor 4 Stimulation in Peripheral Blood Mononuclear Cells from Patients with Recent Onset Rheumatoid Arthritis"],"prefix":"10.1155","volume":"2008","author":[{"given":"M. L.","family":"Kowalski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Wolska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Grzegorczyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Hilt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Jarzebska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Drobniewski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Synder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kurowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,6,23]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780380403"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-199605000-00007"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2004.033779"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00296-003-0316-1"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kei023"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.11.7021"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20219"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200421795"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.10545"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780400104"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2007.03396.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.21951"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113921"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2007.03441.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1155\/MI.2005.343"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.21155"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.47102\/annals-acadmedsg.V36N2p96"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.3109\/03009749009095040"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1996.d01-820.x"},{"key":"e_1_2_6_20_2","first-page":"16","article-title":"Distinct patterns of cytokine secretion characterize new onset synovitis versus \nchronic rheumatoid arthritis","volume":"25","author":"Kanik K. S.","year":"1998","journal-title":"Journal of Rheumatology"},{"key":"e_1_2_6_21_2","first-page":"575","article-title":"Expression of interleukin-1 beta, tumor necrosis factor alpha, interleukins-6, -10 and -4, and metalloproteases by freshly isolated mononuclear cells from early never-treated and non-acute treated rheumatoid arthritis patients","volume":"17","author":"V\u00e1zquez-Del Mercado M.","year":"1999","journal-title":"Clinical & Experimental Rheumatology"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.10148"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780380107"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kem383"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-146-11-200706050-00008"},{"key":"e_1_2_6_26_2","first-page":"623","volume-title":"Oxford Textbook of Rheumatology","author":"Banks E. R.","year":"1998"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kei219"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.162.6.2163"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/322547a0"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.22707"},{"key":"e_1_2_6_31_2","first-page":"1044","article-title":"Arachidonate 15-lipoxygenase of reticulocyte-type in human rheumatoid arthritis type B synoviocytes and modulation of its activity by proinflammatory cytokines","volume":"26","author":"Liagre B.","year":"1999","journal-title":"Journal of Rheumatology"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/33.6.520"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63918-1"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20678"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2005.09.002"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2008\/132732.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2008\/132732.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2008\/132732","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T14:20:37Z","timestamp":1718634037000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2008\/132732"}},"subtitle":[],"editor":[{"given":"Donna-Marie","family":"McCafferty","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2008,1]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,1]]}},"alternative-id":["10.1155\/2008\/132732"],"URL":"https:\/\/doi.org\/10.1155\/2008\/132732","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1]]},"assertion":[{"value":"2007-12-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-03-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-06-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"132732"}}