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Two-dimensional gel electrophoresis and mass spectrometry were employed to separate and identify twenty two Ni-interacting proteins in H. pylori. These Ni-interacting proteins can be classified into several general functional categories, including cellular processes (HspA, HspB, TsaA, and NapA), enzymes (Urease, Fumarase, GuaB, Cad, PPase, and DmpI), membrane-associated proteins (OM jhp1427 and HpaA), iron storage protein (Pfr), and hypothetical proteins (HP0271, HP jhp0216, HP jhp0301, HP0721, HP0614, and HP jhp0118). The implication of these proteins in nickel homeostasis is discussed.<\/jats:p>","DOI":"10.1155\/2008\/289490","type":"journal-article","created":{"date-parts":[[2007,12,13]],"date-time":"2007-12-13T18:43:04Z","timestamp":1197571384000},"page":"1-6","source":"Crossref","is-referenced-by-count":16,"title":["Identification of Proteins Related to Nickel Homeostasis in Helicobater pylori by Immobilized Metal Affinity Chromatography and Two-Dimensional Gel Electrophoresis"],"prefix":"10.1155","volume":"2008","author":[{"given":"Xuesong","family":"Sun","sequence":"first","affiliation":[{"name":"Institute of Life and Health Engineering, Jinan University, Guangzhou 510632, China"},{"name":"Department of Chemistry and Open Laboratory of Chemical Biology, The University of Hong Kong, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiguang","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Open Laboratory of Chemical Biology, The 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