{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:17:37Z","timestamp":1791278257261,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,5,12]],"date-time":"2008-05-12T00:00:00Z","timestamp":1210550400000},"content-version":"vor","delay-in-days":132,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Dutch Diabetes Fund","award":["DFN 92.133"],"award-info":[{"award-number":["DFN 92.133"]}]},{"name":"Dutch Diabetes Fund","award":["14X-6834"],"award-info":[{"award-number":["14X-6834"]}]},{"name":"Dutch Diabetes Fund","award":["72X-4499"],"award-info":[{"award-number":["72X-4499"]}]},{"DOI":"10.13039\/501100006310","name":"Swedish Medical Research Council","doi-asserted-by":"crossref","award":["DFN 92.133"],"award-info":[{"award-number":["DFN 92.133"]}],"id":[{"id":"10.13039\/501100006310","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006310","name":"Swedish Medical Research Council","doi-asserted-by":"crossref","award":["14X-6834"],"award-info":[{"award-number":["14X-6834"]}],"id":[{"id":"10.13039\/501100006310","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006310","name":"Swedish Medical Research Council","doi-asserted-by":"crossref","award":["72X-4499"],"award-info":[{"award-number":["72X-4499"]}],"id":[{"id":"10.13039\/501100006310","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006310","name":"Swedish Medical Research Council","doi-asserted-by":"crossref","award":["DFN 92.133"],"award-info":[{"award-number":["DFN 92.133"]}],"id":[{"id":"10.13039\/501100006310","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006310","name":"Swedish Medical Research Council","doi-asserted-by":"crossref","award":["14X-6834"],"award-info":[{"award-number":["14X-6834"]}],"id":[{"id":"10.13039\/501100006310","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006310","name":"Swedish Medical Research Council","doi-asserted-by":"crossref","award":["72X-4499"],"award-info":[{"award-number":["72X-4499"]}],"id":[{"id":"10.13039\/501100006310","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Albert P\u00e5hlsson","award":["DFN 92.133"],"award-info":[{"award-number":["DFN 92.133"]}]},{"name":"Albert P\u00e5hlsson","award":["14X-6834"],"award-info":[{"award-number":["14X-6834"]}]},{"name":"Albert P\u00e5hlsson","award":["72X-4499"],"award-info":[{"award-number":["72X-4499"]}]},{"name":"NovoNordic and Crafoord Foundations","award":["DFN 92.133"],"award-info":[{"award-number":["DFN 92.133"]}]},{"name":"NovoNordic and Crafoord Foundations","award":["14X-6834"],"award-info":[{"award-number":["14X-6834"]}]},{"name":"NovoNordic and Crafoord Foundations","award":["72X-4499"],"award-info":[{"award-number":["72X-4499"]}]},{"name":"Swedish Diabetes Association","award":["DFN 92.133"],"award-info":[{"award-number":["DFN 92.133"]}]},{"name":"Swedish Diabetes Association","award":["14X-6834"],"award-info":[{"award-number":["14X-6834"]}]},{"name":"Swedish Diabetes Association","award":["72X-4499"],"award-info":[{"award-number":["72X-4499"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Diabetes Research"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:p>Human islet amyloid polypeptide (hIAPP), a pancreatic islet protein of 37 amino acids, is the main component of islet amyloid, seen at autopsy in patients with type 2 diabetes mellitus (DM2). To investigate the roles of hIAPP and islet amyloid in DM2, we generated transgenic mice expressing hIAPP in their islet beta cells. In this study, we found that after a long\u2010term, high\u2010fat diet challenge islet amyloid was observed in only 4 of 19 hIAPP transgenic mice. hIAPP transgenic females exhibited severe glucose intolerance, which was associated with a downregulation of GLUT\u20102 mRNA expression. In isolated islets from hIAPP males cultured for 3 weeks on high\u2010glucose medium, the percentage of amyloid containing islets increased from 5.5% to 70%. This ex vivo system will allow a more rapid, convenient, and specific study of factors influencing islet amyloidosis as well as of therapeutic strategies to interfere with this pathological process.<\/jats:p>","DOI":"10.1155\/2008\/697035","type":"journal-article","created":{"date-parts":[[2008,5,15]],"date-time":"2008-05-15T10:19:33Z","timestamp":1210846773000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Human Islet Amyloid Polypeptide Transgenic Mice:  In Vivo and Ex Vivo Models for the Role of hIAPP in  Type 2 Diabetes Mellitus"],"prefix":"10.1155","volume":"2008","author":[{"given":"J. W. M.","family":"H\u00f6ppener","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. M.","family":"Jacobs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Wierup","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Sotthewes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Sprong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"de Vos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Berger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Sundler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Ahr\u00e9n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,5,12]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.11.3881"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.48.2.241"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200008103430607"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00549079"},{"key":"e_1_2_6_5_2","first-page":"151","article-title":"Islet amyloid, increased A-cells, reduced B-cells and exocrine fibrosis: quantitative changes in the pancreas in type 2 diabetes","volume":"9","author":"Clark A.","year":"1988","journal-title":"Diabetes Research"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1104345"},{"key":"e_1_2_6_7_2","first-page":"1777","article-title":"The insulin and islet amyloid polypeptide genes contain similar cell-specific promoter elements that bind identical B-cell nuclear complexes","volume":"12","author":"German M.","year":"1992","journal-title":"Molecular Cell Biology"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/368756a0"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.48.3.491"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2005.12.009"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0600948103"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.9.2304"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M400348200"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1079469"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00400803"},{"key":"e_1_2_6_16_2","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1530\/eje.0.1360107","article-title":"Biologically active human islet amyloid polypeptide\/amylin in transgenic mice","volume":"136","author":"van Hulst K. L.","year":"1997","journal-title":"European Journal of Endocrinology"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001250051079"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001250051175"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.37.9.1163"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0026-0495(97)90175-X"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.8.3492"},{"key":"e_1_2_6_22_2","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1177\/000456329403100209","article-title":"An improved method for the determination of islet amyloid polypeptide levels in plasma","volume":"31","author":"van Hulst K. L.","year":"1994","journal-title":"Annals of Clinical Biochemistry"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00410276"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90051-7"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-0115(02)00298-7"},{"key":"e_1_2_6_26_2","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1177\/002215540405200204","article-title":"Cocaine- and amphetamine-regulated transcript (CART) is expressed in several islet cell types during rat development","volume":"52","author":"Wierup N.","year":"2004","journal-title":"Journal of Histochemistry & Cytochemistry"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006676-200407000-00063"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00355463"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.47.5.743"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.45.8.1094"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.18.8467"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1390461"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201X.2000.00746.x"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)92529-1"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(91)90239-M"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/335632a0"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(91)91476-S"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.39.2.260"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001250050088"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.41.8.975"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.40.3.395"},{"key":"e_1_2_6_42_2","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0074-7696(08)62677-7","article-title":"Molecular and cellular physiology of GLUT-2, a high-Km facilitated diffusion glucose transporter","volume":"137","author":"Thorens B.","year":"1992","journal-title":"International Review of Cytology"},{"key":"e_1_2_6_43_2","doi-asserted-by":"crossref","first-page":"247","DOI":"10.3109\/13506129809007297","article-title":"Macrophages and pancreatic islet amyloidosis","volume":"5","author":"de Koning E. J. P.","year":"1998","journal-title":"Amyloid"}],"container-title":["Journal of Diabetes Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2008\/697035.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2008\/697035.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2008\/697035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T09:26:00Z","timestamp":1719566760000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2008\/697035"}},"subtitle":[],"editor":[{"given":"Hiroshi","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2008,1]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,1]]}},"alternative-id":["10.1155\/2008\/697035"],"URL":"https:\/\/doi.org\/10.1155\/2008\/697035","archive":["Portico"],"relation":{},"ISSN":["2314-6745","2314-6753"],"issn-type":[{"value":"2314-6745","type":"print"},{"value":"2314-6753","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1]]},"assertion":[{"value":"2007-10-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-02-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-05-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"697035"}}