{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:38:31Z","timestamp":1753886311370,"version":"3.41.2"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T00:00:00Z","timestamp":1213142400000},"content-version":"vor","delay-in-days":162,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:p>HTLV\u20101 causes adult T\u2010cell leukemia (ATL) and HTLV\u20101 associated myelopathy\/tropical spastic paraparesis (HAM\/TSP). Recombinant envelope glycoprotein is used in production of diagnostic enzyme\u2010linked immunosorbent assay (ELISA) kit. There are some reports that a significant percentage of Iranian HTLV\u20101 infected patients showed no seroreactivity with MTA\u20101 peptide, while HTLV\u20101 had been confirmed by PCR detection methods or ELISA kits containing a cocktail of HTLV\u20101 specific peptides. This report describes experiments designed to determine whether some discrepancies between ELISA and PCR results could be due to truncation of immunodominant epitopes using immunoassay method. We have cloned the MTA\u20101 epitope of env gene from HTLV\u20101  in NotI\/NdeI sites of pET22b(+) expression vector. Sequencing analysis of recombinant plasmids revealed an insertion of a cytosine in position 271 causing a stop codon in the MTA\u20101 protein translation. SDS\u2010PAGE analysis also failed to reveal the presence of the desired protein. Subjects with a mutant HTLV\u20101 env gene were shown to be seronegative using ELISA, but positive with PCR.<\/jats:p>","DOI":"10.1155\/2008\/846371","type":"journal-article","created":{"date-parts":[[2008,6,12]],"date-time":"2008-06-12T10:53:36Z","timestamp":1213268016000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Truncated MTA\u20101: A Pitfall in ELISA\u2010Based Immunoassay of HTLV\u20101 Infection"],"prefix":"10.1155","volume":"2008","author":[{"given":"Mohammad Reza","family":"Abbaszadegan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narges","family":"Jafarzadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mojtaba","family":"Sankian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AbdolReza","family":"Varasteh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoud","family":"Mahmoudi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Majid","family":"Sadeghizadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatemeh","family":"Khatami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neema","family":"Mehramiz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,6,11]]},"reference":[{"key":"e_1_2_5_1_2","first-page":"1941","volume-title":"Fields Virology","author":"Gren O. 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