{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T11:56:15Z","timestamp":1774612575997,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,8,5]],"date-time":"2009-08-05T00:00:00Z","timestamp":1249430400000},"content-version":"vor","delay-in-days":216,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Ministry of Science, Technology and Innovation of Malaysia"},{"name":"Malaysian Toray Science Foundation"},{"DOI":"10.13039\/501100004386","name":"Universiti Malaya","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:p>The emergence of <jats:italic>Escherichia coli<\/jats:italic> that produce extended spectrum <jats:italic>\u03b2<\/jats:italic>\u2010lactamases (ESBLs) and are multidrug resistant (MDR) poses antibiotic management problems. Forty\u2010seven <jats:italic>E. coli<\/jats:italic> isolates from various public hospitals in Malaysia were studied. All isolates were sensitive to imipenem whereas 36 were MDR (resistant to 2 or more classes of antibiotics). PCR detection using gene\u2010specific primers showed that 87.5% of the ESBL\u2010producing <jats:italic>E. coli<\/jats:italic> harbored the <jats:italic>b<\/jats:italic><jats:italic>l<\/jats:italic><jats:italic>a<\/jats:italic><jats:sub>TEM<\/jats:sub> gene. Other ESBL\u2010encoding genes detected were <jats:italic>b<\/jats:italic><jats:italic>l<\/jats:italic><jats:italic>a<\/jats:italic><jats:sub>OXA<\/jats:sub>, <jats:italic>b<\/jats:italic><jats:italic>l<\/jats:italic><jats:italic>a<\/jats:italic><jats:sub>SHV<\/jats:sub>, and <jats:italic>b<\/jats:italic><jats:italic>l<\/jats:italic><jats:italic>a<\/jats:italic><jats:sub>CTX-M<\/jats:sub>. Integron\u2010encoded integrases were detected in 55.3% of isolates, with class 1 integron\u2010encoded \n<jats:italic>intI1<\/jats:italic> integrase being the majority. Amplification and sequence analysis of the 5<jats:sup><jats:italic>\u2032<\/jats:italic><\/jats:sup>CS region of the integrons showed known antibiotic resistance\u2010encoding gene cassettes of various sizes \nthat were inserted within the respective integrons. Conjugation and transformation experiments indicated that some of the \nantibiotic resistance genes were likely plasmid\u2010encoded and transmissible. All 47 isolates were subtyped by PFGE and PCR\u2010based fingerprinting using random amplified polymorphic DNA (RAPD), repetitive extragenic palindromes (REPs), and \nenterobacterial repetitive intergenic consensus (ERIC). These isolates were very diverse and heterogeneous. PFGE, ERIC, and \nREP\u2010PCR methods were more discriminative than RAPD in subtyping the <jats:italic>E. coli<\/jats:italic> isolates.<\/jats:p>","DOI":"10.1155\/2009\/165637","type":"journal-article","created":{"date-parts":[[2009,8,5]],"date-time":"2009-08-05T14:59:56Z","timestamp":1249484396000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Characterization of Multidrug Resistant ESBL\u2010Producing <i>Escherichia coli<\/i> Isolates from Hospitals in Malaysia"],"prefix":"10.1155","volume":"2009","author":[{"given":"King-Ting","family":"Lim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohani","family":"Yasin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chew-Chieng","family":"Yeo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Savithri","family":"Puthucheary","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwai-Lin","family":"Thong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,8,5]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki076"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.3142\u20103146.2003"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.1\u201014.2004"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2007.08.003"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168\u20106496(03)00147\u20108"},{"key":"e_1_2_6_6_2","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0255-0857(21)02351-3","article-title":"SHV-5 extended-spectrum \u03b2-lactamases in clinical isolates of Escherichia coli in Malaysia","volume":"24","author":"Subramaniam G.","year":"2006","journal-title":"Indian Journal of Medical Microbiology"},{"key":"e_1_2_6_7_2","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/BF03263188","article-title":"Genetic diversity in Acremonium endophytes isolated from warm-season grasses as revealed by RAPD markers","volume":"13","author":"Latha R.","year":"2004","journal-title":"Journal of Plant Biochemistry and Biotechnology"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.2.362-366.1999"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.10.3113-3117.1999"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.12.3829\u20103836.2002"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4264\u20104269.2003"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.10.3720\u20103728.2004"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01511\u201005"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.5.1823\u20101829.2005"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl505"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh424"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3893\u20103896.2003"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.772\u2010777.2003"},{"key":"e_1_2_6_19_2","doi-asserted-by":"crossref","first-page":"2180","DOI":"10.1128\/aem.58.7.2180-2187.1992","article-title":"Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria","volume":"58","author":"De Bruijn F. 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