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Recently developed nanosensors, (Photonic Explorers for Biomedical use with Biologically Localized Embedding) PEBBLEs, have a number of advantages for measuring ions in cells over established methods using microelectrodes, unbound fluorescent dyes, or NMR. PEBBLE sensors have been shown to work in principle for measuring dynamic ion changes, but few in vivo applications have been demonstrated. We modified the protocol for the fabrication of pH sensing PEBBLEs and developed a protocol for the utilization of these sensors for the monitoring of dynamic pH changes in the endosomes of slime mold <jats:italic>Dictyostelium discoideum (D. discoideum)<\/jats:italic>. Oregon Green 514\u2010CdSe Quantum Dot PEBBLEs were used to measure real\u2010time pH inside <jats:italic>D. discoideum<\/jats:italic> endosomes during cAMP stimulation. Endosomal pH was shown to decrease during cAMP signaling, demonstrating a movement of protons into the endosomes of <jats:italic>D. discoideum<\/jats:italic> amoebae.<\/jats:p>","DOI":"10.1155\/2009\/235158","type":"journal-article","created":{"date-parts":[[2009,8,17]],"date-time":"2009-08-17T16:15:19Z","timestamp":1250525719000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of PEBBLEs as a Tool for Real\u2010Time Measurement of <i>Dictyostelium discoideum<\/i> Endosomal pH"],"prefix":"10.1155","volume":"2009","author":[{"given":"Everett","family":"Moding","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Hellyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Rank","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phoebe","family":"Lostroh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murphy","family":"Brasuel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,8,17]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.149.1.99-105.1982"},{"key":"e_1_2_6_2_2","unstructured":"BozzoneD. 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