{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T07:29:51Z","timestamp":1765438191948,"version":"3.37.3"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2009,1,21]],"date-time":"2009-01-21T00:00:00Z","timestamp":1232496000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI056983"],"award-info":[{"award-number":["R01AI056983"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advances in Bioinformatics"],"published-print":{"date-parts":[[2009,1,21]]},"abstract":"<jats:p>We demonstrate the application and comparative interpretations of three tree-based algorithms for the analysis of data arising from flow cytometry: classification and regression trees (CARTs), random forests (RFs), and logic regression (LR). Specifically, we consider the question of what best predicts CD4 T-cell recovery in HIV-1 infected persons starting antiretroviral therapy with CD4 count between 200 and 350\u2009cell\/<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi>\u03bc<\/mml:mi><\/mml:math>L. A comparison to a more standard contingency table analysis is provided. While contingency table analysis and RFs provide information on the importance of each potential predictor variable, CART and LR offer additional insight into the combinations of variables that together are predictive of the outcome. In all cases considered, baseline CD3-DR-CD56+CD16+ emerges as an important predictor variable, while the tree-based approaches identify additional variables as potentially informative. Application of tree-based methods to our data suggests that a combination of baseline immune activation states, with emphasis on CD8 T-cell activation, may be a better predictor than any single T-cell\/innate cell subset analyzed. Taken together, we show that tree-based methods can be successfully applied to flow cytometry data to better inform and discover associations that may not emerge in the context of a univariate analysis.<\/jats:p>","DOI":"10.1155\/2009\/235320","type":"journal-article","created":{"date-parts":[[2010,1,21]],"date-time":"2010-01-21T15:30:32Z","timestamp":1264087832000},"page":"1-9","source":"Crossref","is-referenced-by-count":8,"title":["Tree-Based Methods for Discovery of Association between Flow Cytometry Data and Clinical Endpoints"],"prefix":"10.1155","volume":"2009","author":[{"given":"M.","family":"Eliot","sequence":"first","affiliation":[{"name":"Division of Biostatistics, University of Massachusetts, Amherst, MA 01003, USA"}]},{"given":"L.","family":"Azzoni","sequence":"additional","affiliation":[{"name":"Immunology Program, Wistar Institute, Philadelphia, PA 19104, USA"}]},{"given":"C.","family":"Firnhaber","sequence":"additional","affiliation":[{"name":"Clinical HIV Research Unit, University of Witwatersrand, Johannesburg, South Africa"}]},{"given":"W.","family":"Stevens","sequence":"additional","affiliation":[{"name":"Department of Hematology and Molecular Medicine, National Health Laboratory Service and University of Witwatersrand, Johannesburg, South Africa"}]},{"given":"D. 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