{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T16:03:16Z","timestamp":1740153796572,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["CA121955","20061478"],"award-info":[{"award-number":["CA121955","20061478"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001159","name":"Hawaii Community Foundation","doi-asserted-by":"publisher","award":["CA121955","20061478"],"award-info":[{"award-number":["CA121955","20061478"]}],"id":[{"id":"10.13039\/100001159","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advances in Hematology"],"published-print":{"date-parts":[[2009]]},"abstract":"<jats:p>Assessing molecular persistent or minimal residual disease (PD\/MRD) in childhood Burkitt lymphoma (BL) is challenging because access to original tumor is usually needed to design patient-specific primers (PSPs). Because BL is characterized by rearranged immunoglobulin heavy chain (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mtext>I<\/mml:mtext><mml:mtext>g<\/mml:mtext><mml:msub><mml:mtext>V<\/mml:mtext><mml:mtext>H<\/mml:mtext><\/mml:msub><\/mml:mrow><\/mml:math>) genes,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mtext>I<\/mml:mtext><mml:mtext>g<\/mml:mtext><mml:msub><mml:mtext>V<\/mml:mtext><mml:mtext>H<\/mml:mtext><\/mml:msub><\/mml:mrow><\/mml:math>primer pools from<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mtext>I<\/mml:mtext><mml:mtext>g<\/mml:mtext><mml:msub><mml:mtext>V<\/mml:mtext><mml:mrow><mml:mtext>H1<\/mml:mtext><\/mml:mrow><\/mml:msub><mml:mrow><mml:mtext>\u2013I<\/mml:mtext><\/mml:mrow><mml:mtext>g<\/mml:mtext><mml:msub><mml:mtext>V<\/mml:mtext><mml:mrow><mml:mtext>H<\/mml:mtext><mml:mn>7<\/mml:mn><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>regions were tested to detect PD\/MRD, thus eliminating the need for original tumor. The focus of the current study was to assess the feasibility of using<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mtext>I<\/mml:mtext><mml:mtext>g<\/mml:mtext><mml:msub><mml:mtext>V<\/mml:mtext><mml:mtext>H<\/mml:mtext><\/mml:msub><\/mml:mrow><\/mml:math>primer pools to detect disease in clinical specimens. Fourteen children diagnosed with B-NHL had follow-up repository specimens available to assess PD\/MRD. Of the 14 patients, 12 were PD\/MRD negative after 2 months of therapy and remained in remission at the end of therapy; 2\/14 patients were PD\/MRD positive at 2-3 months and later relapsed. PSP-based assays from these 14 patients showed 100% concordance with the current assay. This feasibility study warrants further investigation to assess PD\/MRD using<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mtext>I<\/mml:mtext><mml:mtext>g<\/mml:mtext><mml:msub><mml:mtext>V<\/mml:mtext><mml:mtext>H<\/mml:mtext><\/mml:msub><\/mml:mrow><\/mml:math>primer pools, which could have clinical significance as a real-time assessment tool to monitor pediatric BL and possibly other B-cell non-Hodgkin lymphoma therapy.<\/jats:p>","DOI":"10.1155\/2009\/412163","type":"journal-article","created":{"date-parts":[[2009,4,16]],"date-time":"2009-04-16T06:36:07Z","timestamp":1239863767000},"page":"1-7","source":"Crossref","is-referenced-by-count":10,"title":["Screening for Residual Disease in Pediatric Burkitt Lymphoma Using Consensus Primer Pools"],"prefix":"10.1155","volume":"2009","author":[{"given":"Melissa","family":"Agsalda","sequence":"first","affiliation":[{"name":"Department of Cell & Molecular Biology, John A. 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