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Specific miRNA probes were linked to each rotary <jats:italic>\u03b2<\/jats:italic> subunits of <jats:italic>F<\/jats:italic><jats:sub>0<\/jats:sub><jats:italic>F<\/jats:italic><jats:sub>1<\/jats:sub>\u2010ATPase. Detection of miRNAs was based on the proton flux change induced by light\u2010driven rotation of <jats:italic>\u03b4<\/jats:italic>\u2010free <jats:italic>F<\/jats:italic><jats:sub>0<\/jats:sub><jats:italic>F<\/jats:italic><jats:sub>1<\/jats:sub>\u2010ATPase. The hybridization reaction was indicated by changes in the fluorescent intensity of pH\u2010sensitive CdTe quantum dots. Our results showed that the assay was attomole sensitivities (1.2 \u00d7 10<jats:sup>\u221218<\/jats:sup>\u2009mol) to target miRNAs and capable of distinguishing among miRNA family members. Moreover, the method could be used to monitor real\u2010time hybridization without any complicated fabrication before hybridization. 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