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In this investigation, the cytotoxic properties of ZER were evaluated, on cancer cells of human cervix (HeLa), breast and ovary, and normal cells of Chinese Hamster ovary, using MTT assay. Apoptogenic effects of ZER on HeLa were studied using fluorescence microscopy (AO\/PI double staining), scanning and transmission electron microscopy (SEM and TEM), and colorimetric assay of the apoptosis promoter enzyme, caspase\u20103. The results of MTT assay showed that ZER has less effect on normal cells compared to cancer cells. The lowest  IC<jats:sub>50<\/jats:sub> of ZER was observed on HeLa cells. Cytological observations showed nuclear and chromatin condensation, cell shrinkage, multinucleation, abnormalities of mitochondrial cristae, membrane blebbing, holes, cytoplasmic extrusions and formation of apoptotic bodies as confirmed collectively by double staining of AO\/PI, SEM and TEM. Statistical analysis (two\u2010tailed <jats:italic>t<\/jats:italic>\u2010test) of differential counting of 200 cells under fluorescence microscope revealed significant difference in apoptotic cells populations between treated and untreated HeLa cells. In addition, ZER has increased the cellular level of caspase\u20103 on the treated HeLa cells. It could be concluded that ZER was able to produce distinctive morphological features of cell death that corresponds to apoptosis.<\/jats:p>","DOI":"10.1155\/2009\/769568","type":"journal-article","created":{"date-parts":[[2009,3,24]],"date-time":"2009-03-24T14:47:34Z","timestamp":1237906054000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["In Vitro Ultramorphological Assessment of Apoptosis Induced by Zerumbone on (HeLa )"],"prefix":"10.1155","volume":"2009","author":[{"given":"Siddig Ibrahim","family":"Abdel Wahab","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad Bustamam","family":"Abdul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adel Sharaf","family":"Alzubairi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manal","family":"Mohamed Elhassan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syam","family":"Mohan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,3,24]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1211\/jpp.57.12.0009"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20102007\u2010967132"},{"key":"e_1_2_6_3_2","first-page":"181","article-title":"Anti inflammatory property of ethanol and water extracts of Zingiber zerumbet","volume":"35","author":"Somchit N.","year":"2003","journal-title":"Indian Journal of Pharmacology"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475\u20102867\u20107\u20104"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2008.02.038"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1207\/S15327914NC4502_12"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0031\u20109422(00)83937\u20100"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20175"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0024\u20103205(01)01277\u20102"},{"key":"e_1_2_6_10_2","unstructured":"NirmalaD. 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