{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T07:30:21Z","timestamp":1769499021677,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["10-036"],"award-info":[{"award-number":["10-036"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical and Developmental Immunology"],"published-print":{"date-parts":[[2009]]},"abstract":"<jats:p><jats:italic>Helicobacter pylori<\/jats:italic>(<jats:italic>H. pylori<\/jats:italic>) infection is a definite causative factor for gastric ulcers (GUs). In the present study we detected a specific antigen of gastric epithelial cells (HGC-27) using cell ELISA, which was recognized by the sera of GU patients (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>20<\/mml:mn><\/mml:mrow><\/mml:math>) but not in patients with chronic gastritis (CG;<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>20<\/mml:mn><\/mml:mrow><\/mml:math>) or in healthy volunteers (HC;<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>10<\/mml:mn><\/mml:mrow><\/mml:math>). This antigen was over-expressed by a stressful (heat-stressed) environment, and was identified as elongation factor 2 kinase (EF-2K) by western blotting. The GU patients' lymphocytes stimulated by<jats:italic>H. pylori<\/jats:italic>specifically disrupted heat-stressed HGC-27 cells in a cytotoxic assay. In flow cytometry, the effector cells (lymphocytes) from GU patients were significantly differentiated to T helper type 1 lymphocyte (Th1) and cytotoxic T lymphocyte (CTL) as opposed to those from CG patients. The target cells (HGC-27) expressed EF-2K and MHC-class I together with costimulatory molecules from heat stress. This antigen specific immune mechanism could have a prominent role in the pathogenesis of GU.<\/jats:p>","DOI":"10.1155\/2009\/850623","type":"journal-article","created":{"date-parts":[[2009,7,15]],"date-time":"2009-07-15T13:24:39Z","timestamp":1247664279000},"page":"1-10","source":"Crossref","is-referenced-by-count":2,"title":["Immune Reactions Against Elongation Factor 2 Kinase: Specific Pathogenesis of Gastric Ulcer from<i>Helicobacter pylori<\/i>Infection"],"prefix":"10.1155","volume":"2009","author":[{"given":"Kiyoshi","family":"Ayada","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Yokota","sequence":"additional","affiliation":[{"name":"Graduate School of Health Science, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8558, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiro","family":"Kawahara","sequence":"additional","affiliation":[{"name":"Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumiko","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyuki","family":"Hirai","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoki","family":"Inaba","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Kagawa Prefectural Central Hospital, 5-4-16 Ban-cho, Takamatsu, Kagawa 760-8557, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahide","family":"Kita","sequence":"additional","affiliation":[{"name":"Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Okada","sequence":"additional","affiliation":[{"name":"Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhide","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiji","family":"Oguma","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"8","key":"1","doi-asserted-by":"crossref","first-page":"436","DOI":"10.5694\/j.1326-5377.1985.tb113443.x","volume":"142","year":"1985","journal-title":"Medical Journal of Australia"},{"issue":"8","key":"2","doi-asserted-by":"crossref","first-page":"439","DOI":"10.5694\/j.1326-5377.1985.tb113444.x","volume":"142","year":"1985","journal-title":"The Medical Journal of Australia"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1136\/gut.31.2.134"},{"issue":"9","key":"4","doi-asserted-by":"crossref","first-page":"705","DOI":"10.7326\/0003-4819-116-9-705","volume":"116","year":"1992","journal-title":"Annals of Internal Medicine"},{"issue":"16","key":"5","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1056\/NEJM199110173251603","volume":"325","year":"1991","journal-title":"The New England Journal of Medicine"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)92035-Z"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1433"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-199905000-00007"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.130079797"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.9.1.167-175.2002"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-5378.2001.00038.x"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2003.08.028"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2003.02325.x"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27527-0"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46882-0"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45871-0"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.5.2736-2743.2005"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20031208"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.20.100301.064801"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200535050"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200636811"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.51.5.396"},{"issue":"1","key":"23","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1136\/gut.45.1.20","volume":"45","year":"1999","journal-title":"Gut"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.6.1056-1059.2001"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.10.4884"},{"issue":"25","key":"26","doi-asserted-by":"crossref","first-page":"16425","DOI":"10.1016\/S0021-9258(18)55316-3","volume":"266","year":"1991","journal-title":"The Journal of Biological Chemistry"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00676-8"},{"key":"28","first-page":"31","volume":"293, part 1","year":"1993","journal-title":"Biochemical Journal"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(01)00297-7"},{"issue":"9","key":"30","first-page":"1098","volume":"3","year":"2004","journal-title":"Cell Cycle"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.22.6109-6114.2002"}],"container-title":["Clinical and Developmental Immunology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2009\/850623.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2009\/850623.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2009\/850623.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T17:55:47Z","timestamp":1633715747000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jir\/2009\/850623\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009]]},"references-count":31,"alternative-id":["850623","850623"],"URL":"https:\/\/doi.org\/10.1155\/2009\/850623","relation":{},"ISSN":["1740-2522","1740-2530"],"issn-type":[{"value":"1740-2522","type":"print"},{"value":"1740-2530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009]]}}}