{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T22:22:27Z","timestamp":1785450147296,"version":"3.56.0"},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,5,26]],"date-time":"2010-05-26T00:00:00Z","timestamp":1274832000000},"content-version":"vor","delay-in-days":145,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Dermatology Research and Practice"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:p>Pemphigus is a chronic mucocutaneous autoimmune bullous disease that is characterized by loss of cell\u2010cell contact in skin and\/or mucous membranes. Past research has successfully identified desmosomes as immunological targets and has demonstrated that acantholysis is initiated through direct binding of IgG. The exact mechanisms of acantholysis, however, are still missing. Experimental model systems have contributed considerably to today\u2032s knowledge and are still a favourite tool of research. In this paper we will describe to what extent human cell and tissue models represent the in vivo situation, for example, organ cultures of human skin, keratinocyte cultures, and human skin grafted on mice and, furthermore, how suitable they are to study the pathogenesis of pemphigus. Organ cultures closely mimic the architecture of the epidermis but are less suitable to answer posed biochemical questions. Cultured keratinocyte monolayers are convenient in this respect, but their desmosomal make\u2010up in terms of adhesion molecules does not exactly reflect the in vivo situation. Reconstituted skin is a relatively new model that approaches organ culture. In models of human skin grafted on mice, acantholysis can be studied in actual human skin but now with all the advantages of an animal model.<\/jats:p>","DOI":"10.1155\/2010\/143871","type":"journal-article","created":{"date-parts":[[2010,5,26]],"date-time":"2010-05-26T14:33:31Z","timestamp":1274884411000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Experimental Human Cell and Tissue Models of Pemphigus"],"prefix":"10.1155","volume":"2010","author":[{"given":"Gerda","family":"van der Wier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hendri H.","family":"Pas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel F.","family":"Jonkman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,5,26]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-117-29622"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5252"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2007.05.005"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5701248"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2009.01.011"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.339"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M710046200"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1977.tb06141.x"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1965.tb14602.x"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12460978"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00509037"},{"key":"e_1_2_8_12_2","first-page":"442","article-title":"Platelet-derived factors enhance pemphigus acantholysis in skin organ cultures","volume":"64","author":"Hunziker T.","year":"1986","journal-title":"Clinical and Experimental Immunology"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00917138"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12519988"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2003.035"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.070028"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12518278"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200605125"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12513454"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12541618"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12543775"},{"key":"e_1_2_8_22_2","first-page":"345","article-title":"Epidermal acantholysis induced in vitro by pemphigus autoantibody. 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