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To verify the feasibility and advantages of the method, two octahedral symmetrical macromolecules, that is, heat shock protein Degp<jats:sub>24<\/jats:sub> and the Red\u2010cell L Ferritin, were utilized as examples to implement reconstruction by the OSAF method. The schedule for simulation was designed as follows: 2000 random orientated projections of single particles with predefined Euler angles and centers of origins were generated, then different levels of noises that is signal\u2010to\u2010noise ratio (S\/N)  = 0.1, 0.5, and 0.8 were added. The structures reconstructed by the OSAF method were in good agreement with the standard models and the relative errors of the structures reconstructed by the OSAF method to standard structures were very little even for high level noise. The facts mentioned above account for that the OSAF method is feasible and efficient approach to reconstruct structures of macromolecules and have ability to suppress the influence of noise.<\/jats:p>","DOI":"10.1155\/2010\/195274","type":"journal-article","created":{"date-parts":[[2010,1,21]],"date-time":"2010-01-21T15:30:32Z","timestamp":1264087832000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Application of Symmetry Adapted Function Method for Three\u2010Dimensional Reconstruction of Octahedral Biological Macromolecules"],"prefix":"10.1155","volume":"2010","author":[{"given":"Songjun","family":"Zeng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongrong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qibin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,1,21]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_6_1_2","DOI":"10.1038\/386088a0"},{"doi-asserted-by":"publisher","key":"e_1_2_6_2_2","DOI":"10.1038\/386091a0"},{"doi-asserted-by":"publisher","key":"e_1_2_6_3_2","DOI":"10.1016\/S0969\u20102126(00)88348\u20103"},{"doi-asserted-by":"publisher","key":"e_1_2_6_4_2","DOI":"10.1126\/science.288.5467.877"},{"doi-asserted-by":"publisher","key":"e_1_2_6_5_2","DOI":"10.1093\/emboj\/cdf494"},{"doi-asserted-by":"publisher","key":"e_1_2_6_6_2","DOI":"10.1016\/j.str.2004.05.006"},{"doi-asserted-by":"publisher","key":"e_1_2_6_7_2","DOI":"10.1038\/nature06893"},{"doi-asserted-by":"publisher","key":"e_1_2_6_8_2","DOI":"10.1073\/pnas.0711623105"},{"doi-asserted-by":"publisher","key":"e_1_2_6_9_2","DOI":"10.1016\/j.str.2008.02.007"},{"doi-asserted-by":"publisher","key":"e_1_2_6_10_2","DOI":"10.1038\/nature06665"},{"doi-asserted-by":"publisher","key":"e_1_2_6_11_2","DOI":"10.1126\/science.1084155"},{"doi-asserted-by":"publisher","key":"e_1_2_6_12_2","DOI":"10.1016\/S0969\u20102126(03)00091\u20101"},{"doi-asserted-by":"publisher","key":"e_1_2_6_13_2","DOI":"10.1006\/jsbi.1999.4174"},{"doi-asserted-by":"publisher","key":"e_1_2_6_14_2","DOI":"10.1016\/j.jsb.2006.05.004"},{"doi-asserted-by":"publisher","key":"e_1_2_6_15_2","DOI":"10.1006\/jsbi.1996.0003"},{"doi-asserted-by":"publisher","key":"e_1_2_6_16_2","DOI":"10.1016\/j.jsb.2003.09.007"},{"doi-asserted-by":"publisher","key":"e_1_2_6_17_2","DOI":"10.1016\/j.jsb.2007.09.016"},{"doi-asserted-by":"publisher","key":"e_1_2_6_18_2","DOI":"10.1098\/rstb.1971.0054"},{"key":"e_1_2_6_19_2","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0304-3991(88)90016-2","article-title":"Three-dimensional reconstruction from electron micrographs of disordered specimens. 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