{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:14:59Z","timestamp":1785888899066,"version":"3.56.0"},"reference-count":16,"publisher":"Wiley","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p>Protein A of<jats:italic>Staphylococcus aureus<\/jats:italic>is a pathogenic factor whose encoding gene,<jats:italic>spa<\/jats:italic>, shows a variation in length in different strains. In this study the<jats:italic>spa<\/jats:italic>gene variation in<jats:italic>S. aureus<\/jats:italic>isolated from healthy carriers and patients was studied, We also compared this variation among MRSA with MSSA strains. 208 strains of<jats:italic>Staphylococcus aureus<\/jats:italic>which we were isolated from Gorgan, north of Iran were studied, 121 cases from patients and 87 cases from healthy carriers, 59 out of them were<jats:italic>MRSA<\/jats:italic>and 149<jats:italic>MSSA<\/jats:italic>. Samples DNA were extracted and amplified by specific primer of<jats:italic>spa<\/jats:italic>gene. In 4 (3.8%) strains of them no<jats:italic>spa<\/jats:italic>gene was detected, and 10.6% had a dual band (1200 and 1400\u2009bp). In strains with one band, the length of<jats:italic>spa<\/jats:italic>gene differed from 1150 to 1500\u2009bp. The most prevalent length was 1350\u20131400\u2009bp (37%). The frequencies of short<jats:italic>spa<\/jats:italic>bands (1150\u20131200\u2009bp) in patients strains were significantly higher. In 4 (3.8%) strains of them no<jats:italic>spa<\/jats:italic>gene was detected, and 10.6% had a dual band (1200 and 1400\u2009bp). In strains with one band, the length of<jats:italic>spa<\/jats:italic>gene differed from 1150 to 1500\u2009bp. The most prevalent length was 1350\u20131400\u2009bp (37%). The frequencies of short<jats:italic>spa<\/jats:italic>bands (1150\u20131200\u2009bp) in patients strains were significantly higher. The<jats:italic>spa<\/jats:italic>gene length of 1350\u20131400\u2009bp in MSSA was more than in MRSA strains (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>.05<\/mml:mn><\/mml:mrow><\/mml:math>). The average length of<jats:italic>spa<\/jats:italic>in isolated strains from urinary tract infections was more than others. It is concluded that the length of<jats:italic>spa<\/jats:italic>gene depends either on resistance to Methicillin or the source of<jats:italic>S. aureus<\/jats:italic>isolation.<\/jats:p>","DOI":"10.1155\/2010\/351397","type":"journal-article","created":{"date-parts":[[2010,8,31]],"date-time":"2010-08-31T18:34:49Z","timestamp":1283279689000},"page":"1-5","source":"Crossref","is-referenced-by-count":33,"title":["<i>Spa<\/i>Diversity among MRSA and MSSA Strains of<i>Staphylococcus aureus<\/i>in North of Iran"],"prefix":"10.1155","volume":"2010","author":[{"given":"Fatemeh","family":"Shakeri","sequence":"first","affiliation":[{"name":"Islamic Azad University of Lahijan, Lahijan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abolfath","family":"Shojai","sequence":"additional","affiliation":[{"name":"Islamic Azad University of Lahijan, Lahijan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masoud","family":"Golalipour","sequence":"additional","affiliation":[{"name":"Basic Sciences Faculty, Golestan University, Gorgan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somaye","family":"Rahimi Alang","sequence":"additional","affiliation":[{"name":"Islamic Azad University of Lahijan, Lahijan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Vaez","sequence":"additional","affiliation":[{"name":"Golestan University of Medical Sciences, Gorgon 4934174515, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ezzat Allah","family":"Ghaemi","sequence":"additional","affiliation":[{"name":"Golestan University of Medical Sciences, Gorgon 4934174515, Iran"},{"name":"Microbiology Department, Infectious Disease Research Center, Gorgan, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.2002.0533"},{"issue":"1","key":"2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1432-1033.1974.tb03750.x","volume":"48","year":"1974","journal-title":"European Journal of Biochemistry"},{"issue":"3","key":"3","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1016\/S0021-9258(17)43463-6","volume":"259","year":"1984","journal-title":"Journal of Biological Chemistry"},{"issue":"5","key":"4","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1086\/501903","volume":"22","year":"2001","journal-title":"Infection Control and Hospital Epidemiology"},{"issue":"4","key":"5","doi-asserted-by":"crossref","first-page":"250","DOI":"10.7883\/yoken.JJID.2005.250","volume":"58","year":"2005","journal-title":"Japanese Journal of Infectious 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