{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T20:36:19Z","timestamp":1777408579660,"version":"3.51.4"},"reference-count":43,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biomedicine and Biotechnology"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p><jats:italic>Mycobacterium bovis<\/jats:italic>Bacillus Calmette-Gu\u00e9rin (BCG) as a live vector of recombinant bacterial vaccine is a promising system to be used. In this study, we evaluate the disrupted expression of heterologous HIV-1gp120 gene in BCG Pasteur host strain using replicative vectors pMV261 and pJH222. pJH222 carries a lysine complementing gene in BCG lysine auxotrophs. The HIV-1 gp120 gene expression was regulated by BCG hsp60 promoter (in plasmid pMV261) and<jats:italic>Mycobacteria<\/jats:italic>spp.<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi>\u03b1<\/mml:mi><\/mml:math>-antigen promoter (in plasmid pJH222). Among 14 rBCG:HIV-1gp120 (pMV261) colonies screened, 12 showed a partial deletion and two showed a complete deletion. However, deletion was not observed in all 10 rBCG:HIV-1gp120 (pJH222) colonies screened. In this study, we demonstrated that<jats:italic>E. coli<\/jats:italic>\/Mycobacterial expression vectors bearing a weak promoter and lysine complementing gene in a recombinant lysine auxotroph of BCG could prevent genetic rearrangements and disruption of HIV 1gp120 gene expression, a key issue for engineering Mycobacterial based vaccine vectors.<\/jats:p>","DOI":"10.1155\/2010\/357370","type":"journal-article","created":{"date-parts":[[2010,6,27]],"date-time":"2010-06-27T14:35:45Z","timestamp":1277649345000},"page":"1-10","source":"Crossref","is-referenced-by-count":15,"title":["Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in<i>Mycobacterium bovis<\/i>BCG Host Strain: A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors"],"prefix":"10.1155","volume":"2010","author":[{"given":"Joan","family":"Joseph","sequence":"first","affiliation":[{"name":"AIDS Research Unit, Hospital Cl\u00ednic\/IDIBAPS-HIVACAT, University of Barcelona, Calle 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