{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T10:42:28Z","timestamp":1740134548526,"version":"3.37.3"},"reference-count":48,"publisher":"Wiley","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biomedicine and Biotechnology"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p>Stannous chloride (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>SnCl<\/mml:mtext><\/mml:mrow><mml:mn>2<\/mml:mn><\/mml:msub><\/mml:mrow><\/mml:math>) and UVA induce DNA lesions through ROS. The aim of this work was to study the toxicity induced by UVA preillumination, followed by<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>SnCl<\/mml:mtext><\/mml:mrow><mml:mn>2<\/mml:mn><\/mml:msub><\/mml:mrow><\/mml:math>treatment.<jats:italic>E. coli<\/jats:italic>BER mutants were used to identify genes which could play a role in DNA lesion repair generated by these agents. The survival assays showed (i) The<jats:italic>nfo<\/jats:italic>mutant was the most sensitive to<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>SnCl<\/mml:mtext><\/mml:mrow><mml:mn>2<\/mml:mn><\/mml:msub><\/mml:mrow><\/mml:math>; (ii) lethal synergistic effect was observed after UVA pre-illumination, plus<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>SnCl<\/mml:mtext><\/mml:mrow><mml:mn>2<\/mml:mn><\/mml:msub><\/mml:mrow><\/mml:math>incubation, the<jats:italic>nfo<\/jats:italic>mutant being the most sensitive; (iii) wild type and<jats:italic>nfo<\/jats:italic>mutants, transformed with pBW21 plasmid (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mi>n<\/mml:mi><mml:mi>f<\/mml:mi><mml:msup><mml:mi>o<\/mml:mi><mml:mo>+<\/mml:mo><\/mml:msup><\/mml:mrow><\/mml:math>) had their survival increased following treatments. The alkaline agarose gel electrophoresis assays pointed that (i) UVA induced DNA breaks and<jats:italic>fpg<\/jats:italic>mutant was the most sensitive; (ii)<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>SnCl<\/mml:mtext><\/mml:mrow><mml:mn>2<\/mml:mn><\/mml:msub><\/mml:mrow><\/mml:math>-induced DNA strand breaks were higher than those from UVA and<jats:italic>nfo<\/jats:italic>mutant had the slowest repair kinetics; (iii)<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mtext>UVA<\/mml:mtext><mml:mo>+<\/mml:mo><mml:mrow><mml:msub><mml:mrow><mml:mtext>SnCl<\/mml:mtext><\/mml:mrow><mml:mn>2<\/mml:mn><\/mml:msub><\/mml:mrow><\/mml:math>promoted an increase in DNA breaks than<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>SnCl<\/mml:mtext><\/mml:mrow><mml:mn>2<\/mml:mn><\/mml:msub><\/mml:mrow><\/mml:math>and, again,<jats:italic>nfo<\/jats:italic>mutant displayed the slowest repair kinetics. In summary, Nfo protects<jats:italic>E. coli<\/jats:italic>cells against damage induced by<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>SnCl<\/mml:mtext><\/mml:mrow><mml:mn>2<\/mml:mn><\/mml:msub><\/mml:mrow><\/mml:math>and<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mtext>UVA<\/mml:mtext><mml:mo>+<\/mml:mo><mml:mrow><mml:msub><mml:mrow><mml:mtext>SnCl<\/mml:mtext><\/mml:mrow><mml:mn>2<\/mml:mn><\/mml:msub><\/mml:mrow><\/mml:math>.<\/jats:p>","DOI":"10.1155\/2010\/376218","type":"journal-article","created":{"date-parts":[[2010,3,15]],"date-time":"2010-03-15T11:30:04Z","timestamp":1268652604000},"page":"1-9","source":"Crossref","is-referenced-by-count":4,"title":["Endonuclease IV Is the Main Base Excision Repair Enzyme Involved in DNA Damage Induced by UVA Radiation and Stannous Chloride"],"prefix":"10.1155","volume":"2010","author":[{"given":"Ellen S.","family":"Motta","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Radio e Fotobiologia, Departamento de Biof\u00edsica e Biometria, Instituto de Biologia Roberto Alcantara Gomes (IBRAG), Universidade do Estado do Rio de Janeiro (UERJ), Avenue 28 de Setembro, 87, 20551-030 Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Thiago","family":"Souza-Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Radio e Fotobiologia, Departamento de Biof\u00edsica e Biometria, Instituto de Biologia Roberto Alcantara Gomes (IBRAG), Universidade do Estado do Rio de Janeiro (UERJ), Avenue 28 de Setembro, 87, 20551-030 Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuany R.","family":"Cassiano","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Radio e Fotobiologia, Departamento de Biof\u00edsica e Biometria, Instituto de Biologia Roberto Alcantara Gomes (IBRAG), Universidade do Estado do Rio de Janeiro (UERJ), Avenue 28 de Setembro, 87, 20551-030 Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1vio J. 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