{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T05:41:22Z","timestamp":1771911682752,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,7,5]],"date-time":"2010-07-05T00:00:00Z","timestamp":1278288000000},"content-version":"vor","delay-in-days":185,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Biomedical Imaging"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:p>The peak signal or the signal at a predetermined, fixed time point after <jats:sc>D<\/jats:sc>\u2010luciferin injection may be used for the quantitative analysis of in vivo bioluminescence imaging. We repeatedly performed sequential bioluminescence imaging after subcutaneous injection of <jats:sc>D<\/jats:sc>\u2010luciferin in mice bearing subcutaneous tumors. The peak time in each measurement became shorter early after cell inoculation, presumably due to gradual establishment of intratumoral vasculature, and reached a plateau of about 10 min on day 10. Although the correlation between the signal at a fixed time point and the peak signal was high, the signal at 5 or 10 min normalized for the peak signal was lower for earlier days, which caused overestimation of tumor growth. The time course of the signals after <jats:sc>D<\/jats:sc>\u2010luciferin injection may vary with time after cell inoculation, and this variation should be considered when determining the imaging protocol for quantitative bioluminescence tumor monitoring.<\/jats:p>","DOI":"10.1155\/2010\/471408","type":"journal-article","created":{"date-parts":[[2010,7,5]],"date-time":"2010-07-05T14:36:17Z","timestamp":1278340577000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Timing of Imaging after <scp>D<\/scp>\u2010Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging"],"prefix":"10.1155","volume":"2010","author":[{"given":"Yusuke","family":"Inoue","sequence":"first","affiliation":[]},{"given":"Shigeru","family":"Kiryu","sequence":"additional","affiliation":[]},{"given":"Makoto","family":"Watanabe","sequence":"additional","affiliation":[]},{"given":"Arinobu","family":"Tojo","sequence":"additional","affiliation":[]},{"given":"Kuni","family":"Ohtomo","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2010,7,5]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.neo.7900079"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959\u20108049(02)00410\u20100"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.21.12044"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/mthe.2001.0460"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2006.11.006"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2008.08.004"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00259\u2010007\u20100664\u20102"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00259\u2010008\u20101022\u20108"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.2144\/03346st01"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1162\/1535350041464865"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.2310\/7290.2007.00031"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07\u2010103713"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1227\/01.NEU.0000195114.24819.4F"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/bio.1032"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00259\u2010005\u20100048\u20104"}],"container-title":["International Journal of Biomedical Imaging"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijbi\/2010\/471408.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijbi\/2010\/471408.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2010\/471408","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T11:31:03Z","timestamp":1718796663000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2010\/471408"}},"subtitle":[],"editor":[{"given":"Vasilis","family":"Ntziachristos","sequence":"additional","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2010,1]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,1]]}},"alternative-id":["10.1155\/2010\/471408"],"URL":"https:\/\/doi.org\/10.1155\/2010\/471408","archive":["Portico"],"relation":{},"ISSN":["1687-4188","1687-4196"],"issn-type":[{"value":"1687-4188","type":"print"},{"value":"1687-4196","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1]]},"assertion":[{"value":"2010-03-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-05-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-07-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"471408"}}