{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T08:14:29Z","timestamp":1765268069061,"version":"3.41.2"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,3,30]],"date-time":"2010-03-30T00:00:00Z","timestamp":1269907200000},"content-version":"vor","delay-in-days":88,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology","doi-asserted-by":"publisher","award":["17590155"],"award-info":[{"award-number":["17590155"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:p><jats:italic>Background and Aim<\/jats:italic>. Mice with <jats:italic>alymphoplasia<\/jats:italic> (<jats:italic>aly\/aly<\/jats:italic>) mutation characterized by a lack of lymph nodes, Peyer\u2032s patches, and well\u2010defined lymphoid follicles in the spleen were found. In this study, we used splenectomized <jats:italic>aly\/aly<\/jats:italic> mice to elucidate the effects of secondary lymphoid organs in the development of <jats:italic>aly\/aly<\/jats:italic> autoimmune pancreatitis. <jats:italic>Methods<\/jats:italic>. Forty\u2010eight 10\u2010week\u2010old <jats:italic>aly\/aly<\/jats:italic> mice were divided into two groups for splenectomy and sham operation. Histological and immunohistochemical analyses of the pancreas were performed at the ages of 20, 30, and 40 weeks old after operation, respectively. \n<jats:italic>Results<\/jats:italic>. Our results showed that mononuclear cell infiltration was restricted to the interlobular connective tissues at the age of 20 weeks, and not increase obviously at the age of 30 and 40 weeks in splenectomized <jats:italic>aly\/aly<\/jats:italic> mice. Furthermore, an apparent decrease in the expressions of CD4<jats:sup>+<\/jats:sup> T, CD8<jats:sup>+<\/jats:sup> T, and B cells was detected in the pancreatic tissues compared with sham <jats:italic>aly\/aly<\/jats:italic> mice, however, no significant difference in macrophage expression  between mice with and without a splenectomy. \n<jats:italic>Conclusions<\/jats:italic>. Inflammation infiltration and development of the pancreatitis in <jats:italic>aly\/aly<\/jats:italic> mice were suppressed effectively after splenectomy, which was, at least partly, correlated to inhibition of the infiltration of T and B cells in pancreatic tissues but not to macrophages.<\/jats:p>","DOI":"10.1155\/2010\/614890","type":"journal-article","created":{"date-parts":[[2010,3,30]],"date-time":"2010-03-30T15:31:04Z","timestamp":1269963064000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of Splenectomy on Spontaneously Chronic  Pancreatitis in <i>aly\/aly<\/i> Mice"],"prefix":"10.1155","volume":"2010","author":[{"given":"Heng-Xiao","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang-Qin","family":"Yi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayato","family":"Terayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Munekazu","family":"Naito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuichi","family":"Hirai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Qu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nozomi","family":"Yi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Itoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,3,30]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1002\/eji.1830240224","article-title":"A new mutation, aly, that induces a generalized lack of lymph nodes accompanied by immunodeficiency in mice","volume":"24","author":"Miyawaki S.","year":"1994","journal-title":"European Journal of Immunology"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830261129"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/8.7.1067"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830260324"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.092596999"},{"key":"e_1_2_6_6_2","doi-asserted-by":"crossref","first-page":"6711","DOI":"10.4049\/jimmunol.167.12.6711","article-title":"Cutting edge: secondary lymphoid organs are essential for maintaining the CD4, but not CD8, naive T cell pool","volume":"167","author":"Dai Z.","year":"2001","journal-title":"The Journal of Immunology"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/76267"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2005.07.009"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102249.2001.01586.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1159\/000105675"},{"key":"e_1_2_6_11_2","first-page":"1293","article-title":"The critical role of lymph nodes in corneal alloimmunization and graft rejection","volume":"42","author":"Yamagami S.","year":"2001","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102002\u201004\u20101270"},{"key":"e_1_2_6_13_2","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1177\/41.9.7689084","article-title":"A histological processing technique that preserves the integrity of calcified tissues (bone, enamel), yolky amphibian embryos, and growth factor antigens in skeletal tissue","volume":"41","author":"Bourque W. 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