{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:00:47Z","timestamp":1790866847816,"version":"4.1.0"},"reference-count":36,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Foundation of Hebei Province"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biomedicine and Biotechnology"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p><jats:italic>Aim<\/jats:italic>. Investigate the promoter methylation of the Thrombospondin-1 (TSP1) gene in gastric cardia adenocarcinoma (GCA).<jats:italic>Methods<\/jats:italic>. MSP approach, immunohistochemistry method, and RT-PCR were used respectively to examine the promoter methylation of TSP1, its protein and mRNA expression in tumors and corresponding normal tissues. The expression and concentration of TGF-<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi>\u03b2<\/mml:mi><\/mml:math>1 were examined respectively by immunohistochemistry and ELISA method. The status of T cell immunity was examined by Flow cytometry analysis.<jats:italic>Results<\/jats:italic>. TSP1 was methylated in 34\/96 (35.4%) tumor specimens, which was significantly higher than that in corresponding normal tissues (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>.001<\/mml:mn><\/mml:mrow><\/mml:math>). Protein and mRNA expression of TSP1 in GCA tumor tissues were reduced significantly and were associated with TSP1 methylation. The protein expression of TGF-<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi>\u03b2<\/mml:mi><\/mml:math>1 was significantly higher in tumor tissues (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>.001<\/mml:mn><\/mml:mrow><\/mml:math>) and was associated with TNM stage and histological differentiation. The concentration of active and total TGF-<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi>\u03b2<\/mml:mi><\/mml:math>1 did not show significant difference between the GCA patients with hypermethylation of TSP1 and without methylation of TSP1 (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&gt;<\/mml:mo><mml:mn>.05<\/mml:mn><\/mml:mrow><\/mml:math>). The function of T cell immunity was significantly different between the GCA patients with hypermethylation of TSP1 and without methylation of TSP1.<jats:italic>Conclusions<\/jats:italic>. Epigenetic silencing of TSP1 gene by promoter hypermethylation may play an important role in GCA.<\/jats:p>","DOI":"10.1155\/2010\/721485","type":"journal-article","created":{"date-parts":[[2010,3,15]],"date-time":"2010-03-15T15:30:17Z","timestamp":1268667017000},"page":"1-10","source":"Crossref","is-referenced-by-count":16,"title":["Aberrant Methylation of Thrombospondin-1 and Its Association with Reduced Expression in Gastric Cardia Adenocarcinoma"],"prefix":"10.1155","volume":"2010","author":[{"given":"Wei","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Laboratory of Pathology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiming","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Laboratory of Pathology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, 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