{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,11]],"date-time":"2024-08-11T10:30:05Z","timestamp":1723372205240},"reference-count":19,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biomedicine and Biotechnology"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:p>The aim of the study was to investigate the DNA polymorphic genotype in MMP-2 promoter gene as a potential candidate region for the development of the cutaneous T-cell lymphoma (CTCL) and\/or its progression. A total of 89 Czech patients with CTCL (including 23 patients with large plaque parapsoriasis) were compared to 198 controls of similar age and sex distribution, without personal or family history of chronic skin diseases and without personal history of malignancy. The three selected polymorphisms in the promoter of MMP-2 gene (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mo>\u2212<\/mml:mo><\/mml:math>1575<jats:italic>G<\/jats:italic>\/A,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mo>\u2212<\/mml:mo><\/mml:math>1306<jats:italic>C<\/jats:italic>\/T, and<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mo>\u2212<\/mml:mo><\/mml:math>790<jats:italic>T<\/jats:italic>\/G) were determined using the PCR-based methodology with RFLP. In our cohort, the associated<jats:italic>GGCCTT<\/jats:italic>MMP-2 promoter genotype was highly significantly more frequent in CTCL-Ia stage patients compared to patients with parapsoriasis, the tests having high sensitivity and specificity (78%, 83%, resp.). To conclude, use of associated MMP-2 promoter genotype as a DNA marker might make it possible to distinguish between the patients with parapsoriasis and those with CTCL stage Ia, which could substantially improve possibilities of clinical diagnostics, therapy design, and prognosis of this serious condition in the early stages.<\/jats:p>","DOI":"10.1155\/2010\/805907","type":"journal-article","created":{"date-parts":[[2010,6,29]],"date-time":"2010-06-29T14:37:34Z","timestamp":1277822254000},"page":"1-5","source":"Crossref","is-referenced-by-count":8,"title":["Matrix Metalloproteinase-2 Promoter Genotype as a Marker of Cutaneous T-Cell Lymphoma Early Stage"],"prefix":"10.1155","volume":"2010","author":[{"given":"Anna","family":"Vasku","sequence":"first","affiliation":[{"name":"Department of Pathological Physiology, Faculty of Medicine, Masaryk University, University Campus Bohunice, A18, Kamenice 5, 62500 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie Bienertova","family":"Vasku","sequence":"additional","affiliation":[{"name":"Department of Pathological Physiology, Faculty of Medicine, Masaryk University, University Campus Bohunice, A18, Kamenice 5, 62500 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miroslav","family":"Ne\u010das","sequence":"additional","affiliation":[{"name":"First Department of Dermatovenerology, St. Ann's Faculty Affiliated Hospital, Masaryk University, 65691 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Vasku","sequence":"additional","affiliation":[{"name":"First Department of Dermatovenerology, St. Ann's Faculty Affiliated Hospital, Masaryk University, 65691 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"98","reference":[{"issue":"3","key":"1","first-page":"1945","volume":"19","year":"1999","journal-title":"Anticancer Research"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1518"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(97)00186-X"},{"issue":"2\u20134","key":"4","first-page":"77","volume":"7","year":"2000","journal-title":"Developmental Immunology"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0532"},{"issue":"19","key":"6","first-page":"5543","volume":"62","year":"2002","journal-title":"Cancer Research"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-3570"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2001.117722"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2003.10.004"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.4103\/0378-6323.51239"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200001000-00005"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-0197"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211536200"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M010242200"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209696"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1038\/11926"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00063-09"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.12.007"},{"issue":"8","key":"19","first-page":"619","volume":"145","year":"2006","journal-title":"Casopis Lekaru Ceskych"}],"container-title":["Journal of Biomedicine and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2010\/805907.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2010\/805907.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2010\/805907.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2016,8,19]],"date-time":"2016-08-19T02:43:33Z","timestamp":1471574613000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2010\/805907\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":19,"alternative-id":["805907","805907"],"URL":"https:\/\/doi.org\/10.1155\/2010\/805907","relation":{},"ISSN":["1110-7243","1110-7251"],"issn-type":[{"value":"1110-7243","type":"print"},{"value":"1110-7251","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010]]}}}