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MicroRNAs (miRNA) have been reported to play essential roles in cell proliferation, cell differentiation, and pathogenesis of human diseases including viral infections. We analyzed the miRNA expression profiles from adenovirus type 3 (AD3) infected Human laryngeal epithelial (Hep2) cells using a SOLiD deep sequencing. 492 precursor miRNAs were identified in the AD3 infected Hep2 cells, and 540 precursor miRNAs were identified in the control. A total of 44 miRNAs demonstrated high expression and 36 miRNAs showed lower expression in the AD3 infected cells than control. The biogenesis of miRNAs has been analyzed, and some of the SOLiD results were confirmed by Quantitative PCR analysis. The present studies may provide a useful clue for the biological function research into AD3 infection.<\/jats:p>","DOI":"10.1155\/2010\/915980","type":"journal-article","created":{"date-parts":[[2010,6,9]],"date-time":"2010-06-09T10:34:48Z","timestamp":1276079688000},"page":"1-8","source":"Crossref","is-referenced-by-count":27,"title":["High-Throughput Sequencing of MicroRNAs in Adenovirus Type 3 Infected Human Laryngeal Epithelial Cells"],"prefix":"10.1155","volume":"2010","author":[{"given":"Yuhua","family":"Qi","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, SiPaiLou2#, Nanjing 210096, China"},{"name":"Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health, Microbiological Laboratory, Jiangsu Center for Disease Prevention and Control (CDC), 172 Jiangsu Rd, Nanjing 210009, 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