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The arenavirus Jun\u00edn (JUNV) is the etiological agent of Argentine Hemorrhagic Fever, an acute disease with high mortality rate. It has been proposed that Z is the functional counterpart of the matrix proteins found in other negative-stranded enveloped RNA viruses. Here we report the optimized expression of a synthetic gene of Z protein, using three expression systems (two bacterial and a baculoviral one). One of these recombinant proteins was used to generate antibodies. A bioinformatic analysis was made where Z was subdivided into three domains. The data presented contributes methodologies for Z recombinant production and provides the basis for the development of new experiments to test its function.<\/jats:p>","DOI":"10.1155\/2010\/970491","type":"journal-article","created":{"date-parts":[[2010,6,29]],"date-time":"2010-06-29T14:37:35Z","timestamp":1277822255000},"page":"1-14","source":"Crossref","is-referenced-by-count":4,"title":["Expression and Purification of Z Protein from Jun\u00edn Virus"],"prefix":"10.1155","volume":"2010","author":[{"given":"S. E.","family":"Go\u00f1i","sequence":"first","affiliation":[{"name":"Departamento de Ciencia y Tecnolog\u00eda, Universidad Nacional de Quilmes, LIGBCM, Roque S\u00e1enz Pe\u00f1a 352, CP B1876BXD, Bernal, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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