{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:43:00Z","timestamp":1760888580114,"version":"3.41.2"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,10,10]],"date-time":"2011-10-10T00:00:00Z","timestamp":1318204800000},"content-version":"vor","delay-in-days":282,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:p>Confocal light absorption and scattering spectroscopic (CLASS) microscopy can detect changes in biochemicals and the morphology of cells. It is therefore used to detect high\u2010grade cervical squamous intraepithelial lesion (HSIL) cells in the diagnosis of premalignant cervical lesions. Forty cervical samples from women with abnormal Pap smear test results were collected, and twenty cases were diagnosed as HSIL; the rest were normal or low\u2010grade cervical squamous intraepithelial lesion (LSIL). The enlarged and condensed nuclei of HSIL cells as viewed under CLASS microscopy were much brighter and bigger than those of non\u2010HSIL cells. Cytological elastic scattered light data was then collected at wavelengths between 400 and 1000\u2009nm. Between 600\u2009nm to 800\u2009nm, the relative elastic scattered light intensity of HSIL cells was higher than that of the non\u2010HSIL. Relative intensity peaks occurred at 700\u2009nm and 800\u2009nm. CLASS sensitivity and specificity results for HSIL and non\u2010HSIL compared to cytology diagnoses were 80% and 90%, respectively. This study demonstrated that CLASS microscopy could effectively detect cervical precancerous lesions. Further study will verify this conclusion before the method is used in clinic for early detection of cervical cancer.<\/jats:p>","DOI":"10.1155\/2011\/214781","type":"journal-article","created":{"date-parts":[[2011,10,10]],"date-time":"2011-10-10T20:27:08Z","timestamp":1318278428000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Separation of Normal and Premalignant Cervical Epithelial Cells Using Confocal Light Absorption and Scattering Spectroscopic Microscopy <i>Ex Vivo<\/i>"],"prefix":"10.1155","volume":"2011","author":[{"given":"Ling","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Tao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Ping","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da-Ren","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,10,10]]},"reference":[{"key":"e_1_2_6_1_2","unstructured":"NCCC National Cervical Cancer Coalition 2011 http:\/\/www.nccc-online.org\/."},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.18553\/jmcp.2010.16.3.217"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-132-10-200005160-00009"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.grf.0000151582.70615.06"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1364\/AO.46.001760"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2007.01.001"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/35017638"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.80.627"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.429979"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.1528950"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1364\/AO.37.003586"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.3802\/jgo.2009.20.2.72"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2011\/214781.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2011\/214781.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2011\/214781","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T11:06:49Z","timestamp":1720091209000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2011\/214781"}},"subtitle":[],"editor":[{"given":"Manoor Prakash","family":"Hande","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2011,1]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["10.1155\/2011\/214781"],"URL":"https:\/\/doi.org\/10.1155\/2011\/214781","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"type":"print","value":"2314-6133"},{"type":"electronic","value":"2314-6141"}],"subject":[],"published":{"date-parts":[[2011,1]]},"assertion":[{"value":"2011-04-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-07-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-10-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"214781"}}