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Anesthetized mice underwent femur fracture\/sublethal hemorrhage and cecal ligation and puncture (CLP) 48\u2009h later. To monitor outcome\u2010dependent changes in circulating cells\/biomarkers, mice were sampled daily (facial vein) for 7 days and retrospectively divided into either dead (DIE) or surviving (SUR) by post\u2010CLP day 7. Prior to CLP, AST was 3\u2010fold higher in DIE, while all other post\u2010TH changes were similar between groups. There was a significant post\u2010CLP intergroup separation. In SUR, RBC and Hb were lower, platelets and neutrophils higher, and lymphocytes mixed compared to DIE. In DIE, all organ function markers except glucose (decrease) were few folds higher compared to SUR. In summary, the combination of daily monitoring with an adequate two\u2010hit model simulates the ICU setting, allows insight into outcome\u2010based responses, and can identify biomarkers indicative of death in the acute posttraumatic sepsis in mice.<\/jats:p>","DOI":"10.1155\/2011\/357926","type":"journal-article","created":{"date-parts":[[2011,1,18]],"date-time":"2011-01-18T19:39:01Z","timestamp":1295379541000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Experimentally Approaching the ICU: Monitoring Outcome\u2010Based Responses in the Two\u2010Hit Mouse Model of Posttraumatic Sepsis"],"prefix":"10.1155","volume":"2011","author":[{"given":"Susanne","family":"Drechsler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrin M.","family":"Weixelbaumer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinz","family":"Redl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martijn","family":"van Griensven","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soheyl","family":"Bahrami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcin F.","family":"Osuchowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,1,18]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200107000-00002"},{"key":"e_1_2_7_2_2","first-page":"293","article-title":"EPIC II: sepsis around the world","volume":"74","author":"Vincent J. 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