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Therefore, the advantage of combined use of high\u2010pressure liquid chromatography (HPLC), mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy will be shown analyzing three different types of extracts of the ubiquitous membrane component phosphatidylcholine. At first, different reversed phase modifications were tested on phosphatidylcholines (PC) with the same effective carbon number (ECN) for their applicability in lipid analysis. The results were taken to improve the separation of three natural PC extract types and a new reversed phase (RP)\u2010HPLC method was developed. The individual species were characterized by one\u2010 and two\u2010dimensional NMR and positive or negative ion mode quadrupole time of flight (q\u2010TOF)\u2010MS as well as MS\/MS techniques. Furthermore, ion suppression effects during electrospray ionisation (ESI), difficulties, limits, and advantages of the individual analytical techniques are addressed.<\/jats:p>","DOI":"10.1155\/2011\/385786","type":"journal-article","created":{"date-parts":[[2010,8,25]],"date-time":"2010-08-25T18:34:39Z","timestamp":1282761279000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Combined Reversed Phase HPLC, Mass Spectrometry, and NMR Spectroscopy for a Fast Separation and Efficient Identification of 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