{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:08:23Z","timestamp":1740103703373,"version":"3.37.3"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Infectious Diseases in Obstetrics and Gynecology"],"published-print":{"date-parts":[[2011]]},"abstract":"<jats:p>Presence of<jats:italic>Chlamydia trachomatis<\/jats:italic>in endocervix was determined in 2466 women attending a tertiary care hospital in New Delhi, India over a period of 16 years, using a monoclonal-based direct immunofluorescence assay, tissue culture isolation, and a conventional PCR assay.<jats:italic>Chlamydia<\/jats:italic>antigen could be detected in 391 out of 2466 (15.85%) of patients studied; in 27.27% women with PID, 16.74% women with cervicitis, 16.03% women with infertility, and 12.06% women with adverse pregnancy outcomes, respectively. There was a statistically significant decreasing trend in<jats:italic>Chlamydia<\/jats:italic>antigen positivity between the years 1994\u20131999 and 2000\u20132004; the apparent decline in antigen positivity between the years 2000\u20132004 and 2005\u20132010 was not statistically significant. Antigen detection assay detected equal number of positives as the PCR assay; tissue culture isolation demonstrated lower positivity. In a few representative specimens from cervicitis patients, genotyping was done using RFLP pattern analysis of<jats:italic>C. trachomatis<\/jats:italic>MOMP gene amplified by PCR assay, all of these belonged to<jats:italic>Chlamydia trachomatis<\/jats:italic>serovar E.<\/jats:p>","DOI":"10.1155\/2011\/548219","type":"journal-article","created":{"date-parts":[[2011,6,26]],"date-time":"2011-06-26T21:40:40Z","timestamp":1309124440000},"page":"1-6","source":"Crossref","is-referenced-by-count":9,"title":["<i>C. trachomatis<\/i>in Female Reproductive Tract Infections and RFLP-Based Genotyping: A 16-Year Study from a Tertiary Care Hospital"],"prefix":"10.1155","volume":"2011","author":[{"given":"Satpathy","family":"Gita","sequence":"first","affiliation":[{"name":"Department of Ocular Microbiology, Dr R. P. Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mittal","family":"Suneeta","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharma","family":"Anjana","sequence":"additional","affiliation":[{"name":"Department of Ocular Microbiology, Dr R. P. Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nayak","family":"Niranjan","sequence":"additional","affiliation":[{"name":"Department of Ocular Microbiology, Dr R. P. 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