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We have reported an expanded pool of CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>high<\/jats:sup>T<jats:sub>reg<\/jats:sub>cells in patients with chronic lymphatic leukemia (CLL), multiple myeloma (MM) as well as its premalignant precursor monoclonal gammopathy of undetermined significance (MGUS). In healthy individuals, low-level expression of CD127 on T cells in addition to the expression of FOXP3 has been associated with T<jats:sub>reg<\/jats:sub>cells. Here, we demonstrate that the expanded FOXP3<jats:sup>+<\/jats:sup>T-cell population in patients with colorectal cancer, CLL, MGUS, MM, follicular lymphoma, and Hodgkin's disease are exclusively CD127<jats:sup>low<\/jats:sup>T<jats:sub>reg<\/jats:sub>cells and were strongly suppressive. A significant portion of CD127<jats:sup>low<\/jats:sup>FOXP3<jats:sup>+<\/jats:sup>T<jats:sub>reg<\/jats:sub>cells expressed only low levels of CD25 suggesting that the previously reported expansion of CD25<jats:sup>+<\/jats:sup>T<jats:sub>reg<\/jats:sub>cells underestimates the true expansion. The assessment of CCR7 and CD45RA expression on the expanded CD4<jats:sup>+<\/jats:sup>CD127<jats:sup>low<\/jats:sup>FOXP3<jats:sup>+<\/jats:sup>T<jats:sub>reg<\/jats:sub>cells revealed an increase of both na\u00efve as well as central and effector memory T<jats:sub>reg<\/jats:sub>cells in peripheral blood. Our data strongly support superiority of combined CD127 and FOXP3 analysis in comparison to CD25 and FOXP3 assessment for further quantification of T<jats:sub>reg<\/jats:sub>cells in malignant diseases.<\/jats:p>","DOI":"10.1155\/2011\/734036","type":"journal-article","created":{"date-parts":[[2011,8,28]],"date-time":"2011-08-28T15:00:49Z","timestamp":1314543649000},"page":"1-12","source":"Crossref","is-referenced-by-count":26,"title":["Comparative Approach to Define Increased Regulatory T Cells in Different Cancer Subtypes by Combined Assessment of CD127 and FOXP3"],"prefix":"10.1155","volume":"2011","author":[{"given":"Marc","family":"Beyer","sequence":"first","affiliation":[{"name":"Laboratory for Genomics and Immunoregulation, LIMES-Institute, University of Bonn, Carl-Troll-Street 31, 53115 Bonn, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Classen","sequence":"additional","affiliation":[{"name":"Laboratory for Genomics and 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