{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T02:28:46Z","timestamp":1761964126563,"version":"build-2065373602"},"reference-count":72,"publisher":"Wiley","license":[{"start":{"date-parts":[[2011,7,14]],"date-time":"2011-07-14T00:00:00Z","timestamp":1310601600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Signal Transduction"],"published-print":{"date-parts":[[2011,7,14]]},"abstract":"<jats:p>Production of RBCs is triggered by the action of erythropoietin (Epo) through its binding to surface receptors (Epo-R) on erythroid precursors in the bone marrow. The intensity and the duration of the Epo signal are regulated by several factors, including the balance between the activities of kinesase and phosphatases. The Epo signal determines the proliferation and maturation of the precursors into hemoglobin (Hb)-containing RBCs. The activity of various protein tyrosine phosphatases, including those involved in the Epo pathway, can be inhibited by sodium orthovanadate (Na<jats:sub>3<\/jats:sub>VO<jats:sub>4<\/jats:sub>, vanadate). Adding vanadate to cultured erythroid precursors of normal donors and patients with<jats:italic>\u03b2<\/jats:italic>-thalassemia enhanced cell proliferation and arrested maturation. This was associated with an increased production of fetal hemoglobin (HbF). Increased HbF in patients with<jats:italic>\u03b2<\/jats:italic>-hemoglobinopathies (<jats:italic>\u03b2<\/jats:italic>-thalassemia and sickle cell disease) ameliorates the clinical symptoms of the disease. These results raise the possibility that specific and nontoxic inhibitors of phosphatases may be considered as a therapeutic modality for elevating HbF in patients with<jats:italic>\u03b2<\/jats:italic>-hemoglobinopathies as well as for intensifying the Epo response in other forms of anemia.<\/jats:p>","DOI":"10.1155\/2011\/860985","type":"journal-article","created":{"date-parts":[[2011,7,14]],"date-time":"2011-07-14T21:41:13Z","timestamp":1310679673000},"page":"1-7","source":"Crossref","is-referenced-by-count":9,"title":["Involvement of Phosphatases in Proliferation, Maturation, and Hemoglobinization of Developing Erythroid Cells"],"prefix":"10.1155","volume":"2011","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6658-6126","authenticated-orcid":true,"given":"Eitan","family":"Fibach","sequence":"first","affiliation":[{"name":"Department of Hematology, Hadassah-Hebrew University Medical Center, Jerusalem 91120, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"2","key":"1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1152\/physrev.1992.72.2.449","volume":"72","year":"1992","journal-title":"Physiological Reviews"},{"issue":"12","key":"2","doi-asserted-by":"crossref","first-page":"2583","DOI":"10.1182\/blood.V77.12.2583.2583","volume":"77","year":"1991","journal-title":"Blood"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.9.5415"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(98)00101-5"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90190-N"},{"issue":"5","key":"6","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/0092-8674(95)90351-8","volume":"80","year":"1995","journal-title":"Cell"},{"issue":"11","key":"7","doi-asserted-by":"crossref","first-page":"4495","DOI":"10.1182\/blood.V87.11.4495.bloodjournal87114495","volume":"87","year":"1996","journal-title":"Blood"},{"issue":"2","key":"8","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1182\/blood.V81.2.328.328","volume":"81","year":"1993","journal-title":"Blood"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1038\/35053086"},{"issue":"51","key":"10","doi-asserted-by":"crossref","first-page":"47771","DOI":"10.1074\/jbc.C100583200","volume":"276","year":"2001","journal-title":"Journal of Biological Chemistry"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)00697-8"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.6.4398"},{"issue":"11","key":"13","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1101\/gad.12.11.1610","volume":"12","year":"1998","journal-title":"Genes and Development"},{"key":"14","first-page":"87","volume":"149","year":"2003","journal-title":"Reviews of Physiology, Biochemistry and Pharmacology"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2008.04.003"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2009.09.001"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1997.d01-2071.x"},{"issue":"3","key":"18","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1111\/j.1432-1033.1997.t01-1-00637.x","volume":"249","year":"1997","journal-title":"European Journal of Biochemistry"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2005.03.023"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.2741\/3051"},{"issue":"1","key":"21","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1111\/j.1365-2141.1994.tb04974.x","volume":"88","year":"1994","journal-title":"British Journal of Haematology"},{"key":"22","first-page":"391","volume":"327, part 2","year":"1997","journal-title":"Biochemical Journal"},{"issue":"22","key":"23","doi-asserted-by":"crossref","first-page":"8864","DOI":"10.1021\/bi00448a027","volume":"28","year":"1989","journal-title":"Biochemistry"},{"issue":"4968","key":"24","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1126\/science.2166336","volume":"249","year":"1990","journal-title":"Science"},{"issue":"5","key":"25","doi-asserted-by":"crossref","first-page":"2896","DOI":"10.1016\/S0021-9258(19)39885-0","volume":"265","year":"1990","journal-title":"Journal of Biological Chemistry"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.21.10306"},{"issue":"8","key":"27","doi-asserted-by":"crossref","first-page":"5886","DOI":"10.1016\/S0021-9258(18)53403-7","volume":"268","year":"1993","journal-title":"Journal of Biological Chemistry"},{"issue":"38","key":"28","doi-asserted-by":"crossref","first-page":"23407","DOI":"10.1016\/S0021-9258(17)31530-2","volume":"269","year":"1994","journal-title":"Journal of Biological Chemistry"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.36.20952"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.36.22251"},{"issue":"4","key":"31","first-page":"500","volume":"20","year":"1992","journal-title":"Experimental Hematology"},{"issue":"6","key":"32","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1182\/blood.V96.6.2084","volume":"96","year":"2000","journal-title":"Blood"},{"issue":"10","key":"33","doi-asserted-by":"crossref","first-page":"3574","DOI":"10.1182\/blood.V89.10.3574","volume":"89","year":"1997","journal-title":"Blood"},{"issue":"5","key":"34","first-page":"654","volume":"232","year":"2007","journal-title":"Experimental Biology and Medicine"},{"issue":"1","key":"35","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1182\/blood.V73.1.100.100","volume":"73","year":"1989","journal-title":"Blood"},{"issue":"10","key":"36","doi-asserted-by":"crossref","first-page":"3624","DOI":"10.1182\/blood.V96.10.3624","volume":"96","year":"2000","journal-title":"Blood"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-12-126938"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e32832990a4"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24141"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.2174\/156652408786241384"},{"issue":"3","key":"41","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1111\/j.1600-0609.1986.tb02301.x","volume":"37","year":"1986","journal-title":"Scandinavian Journal of Haematology"},{"year":"2001","key":"42"},{"issue":"9","key":"43","first-page":"1141","volume":"20","year":"1992","journal-title":"Experimental Hematology"},{"issue":"593","key":"44","first-page":"679","volume":"1","year":"1968","journal-title":"British Medical Journal"},{"key":"45","first-page":"200","volume":"7","year":"1979","journal-title":"Experimental Hematology"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcibr1010767"},{"issue":"4","key":"47","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1080\/02724936.1986.11748452","volume":"6","year":"1986","journal-title":"Annals of Tropical Paediatrics"},{"issue":"3","key":"48","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1136\/jmg.14.3.168","volume":"14","year":"1977","journal-title":"Journal of Medical Genetics"},{"issue":"9","key":"49","doi-asserted-by":"crossref","first-page":"5130","DOI":"10.1073\/pnas.77.9.5130","volume":"77","year":"1980","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"5214","key":"50","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1126\/science.7539154","volume":"268","year":"1995","journal-title":"Science"},{"issue":"2","key":"51","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1056\/NEJM198801143180207","volume":"318","year":"1988","journal-title":"The New England Journal of Medicine"},{"issue":"2","key":"52","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1111\/j.1365-2141.1995.tb05288.x","volume":"91","year":"1995","journal-title":"British Journal of Haematology"},{"issue":"15","key":"53","doi-asserted-by":"crossref","first-page":"4842","DOI":"10.1073\/pnas.80.15.4842","volume":"80","year":"1983","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"5","key":"54","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1182\/blood.V90.5.2075","volume":"90","year":"1997","journal-title":"Blood"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(96)00476-5"},{"issue":"6","key":"56","first-page":"1999","volume":"75","year":"2005","journal-title":"The Journal of Clinical Investigation"},{"issue":"2","key":"57","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1172\/JCI111731","volume":"75","year":"1985","journal-title":"The Journal of Clinical Investigation"},{"issue":"1","key":"58","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1182\/blood.V63.1.201.201","volume":"63","year":"1984","journal-title":"Blood"},{"issue":"1","key":"59","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/14767059909063144","volume":"8","year":"1999","journal-title":"Journal of Maternal-Fetal and Neonatal Medicine"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1007\/s002770100317"},{"issue":"3","key":"61","first-page":"74","volume":"19","year":"1992","journal-title":"Seminars in Oncology"},{"issue":"12","key":"62","doi-asserted-by":"crossref","first-page":"3258","DOI":"10.1182\/blood.V80.12.3258.3258","volume":"80","year":"1992","journal-title":"Blood"},{"issue":"4","key":"63","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1182\/blood.V78.4.1145.1145","volume":"78","year":"1991","journal-title":"Blood"},{"issue":"7","key":"64","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1056\/NEJM198708133170704","volume":"317","year":"1987","journal-title":"The New England Journal of Medicine"},{"key":"65","first-page":"269","volume":"316b","year":"1989","journal-title":"Progress in Clinical and Biological Research"},{"issue":"10","key":"66","doi-asserted-by":"crossref","first-page":"2967","DOI":"10.1182\/blood.V85.10.2967.bloodjournal85102967","volume":"85","year":"1995","journal-title":"Blood"},{"key":"67","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1599"},{"issue":"7","key":"68","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.1182\/blood.V79.7.1861.1861","volume":"79","year":"1992","journal-title":"Blood"},{"issue":"2","key":"69","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1182\/blood.V86.2.572.bloodjournal862572","volume":"86","year":"1995","journal-title":"Blood"},{"key":"70","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-07-170712"},{"key":"71","doi-asserted-by":"publisher","DOI":"10.1007\/BF01075941"},{"key":"72","doi-asserted-by":"publisher","DOI":"10.1210\/jc.80.11.3311"}],"container-title":["Journal of Signal Transduction"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/archive\/2011\/860985.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/archive\/2011\/860985.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/archive\/2011\/860985.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T18:56:26Z","timestamp":1712602586000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jst\/2011\/860985\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7,14]]},"references-count":72,"alternative-id":["860985","860985"],"URL":"https:\/\/doi.org\/10.1155\/2011\/860985","relation":{},"ISSN":["2090-1739","2090-1747"],"issn-type":[{"type":"print","value":"2090-1739"},{"type":"electronic","value":"2090-1747"}],"subject":[],"published":{"date-parts":[[2011,7,14]]}}}