{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:08:08Z","timestamp":1761394088380},"reference-count":39,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Infectious Diseases in Obstetrics and Gynecology"],"published-print":{"date-parts":[[2011]]},"abstract":"<jats:p>Nucleic acids of human papillomavirus (HPV) isolated by manual extraction method (AmpliLute) and automated MagNA pure system were compared and evaluated with cytohistological findings in 253 women. The concordance level between AmpliLute and MagNA was very good 93.3% (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi>\u03ba<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.864<\/mml:mn><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>.0001<\/mml:mn><\/mml:math>). Overall HPVpositivity detected by AmpliLute was 57.3% (30.4% as single and 27% as multiple infections) in contrast to MagNA 54.5% (32% and 23%, resp.). Discrepant results observed in 25 cases: 11 MagNA(\u2212)\/AmpliLute(+), 10 of which had positive histology; 5 MagNA(+)\/AmpliLute(\u2212) with negative histology; 8 MagNA(+)\/AmpliLute(+): in 7 of which AmpliLute detected extra HPV genotypes and 1 MagNA(invalid)\/AmpliLute(+) with positive histology. Both methods performed well when compared against cytological (area under curve (AUC) of AmpliLute 0.712 versus 0.672 of MagNA) and histological diagnoses (AUC of AmpliLute 0.935 versus 0.877 of MagNA), with AmpliLute showing a slightly predominance over MagNA. However, higher sensitivities, specificities, and positive\/negative predictive values were obtained by AmpliLute.<\/jats:p>","DOI":"10.1155\/2011\/931281","type":"journal-article","created":{"date-parts":[[2011,9,21]],"date-time":"2011-09-21T07:55:44Z","timestamp":1316591744000},"page":"1-11","source":"Crossref","is-referenced-by-count":8,"title":["Performance Evaluation of Manual and Automated (MagNA Pure) Nucleic Acid Isolation in HPV Detection and Genotyping Using Roche Linear Array HPV Test"],"prefix":"10.1155","volume":"2011","author":[{"given":"Aikaterini","family":"Chranioti","sequence":"first","affiliation":[{"name":"Department of Diagnostic Cytopathology, University General Hospital \u201cAttikon\u201d, Rimini 1, Chaidari, 12462 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evangelia","family":"Aga","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Cytopathology, University General Hospital \u201cAttikon\u201d, Rimini 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