{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:03:27Z","timestamp":1762862607620,"version":"3.41.2"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,12,12]],"date-time":"2011-12-12T00:00:00Z","timestamp":1323648000000},"content-version":"vor","delay-in-days":345,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:p><jats:italic>Clostridium botulinum<\/jats:italic> is a spore\u2010forming bacterium that can produce a very powerful neurotoxin that causes botulism. In this study, we have investigated the Fur transcription regulators in <jats:italic>Clostridium botulinum<\/jats:italic> and Fur\u2010regulated genes in <jats:italic>Clostridium botulinum<\/jats:italic> A ATCC 3502. We found that gene loss may be the main cause leading to the different numbers of Fur transcription regulators in different <jats:italic>Clostridium botulinum<\/jats:italic> strains. Meanwhile, 46 operons were found to be regulated by the Fur transcription regulator in <jats:italic>Clostridium botulinum<\/jats:italic> A ATCC 3502, involved in several functional classifications, including iron acquisition, iron utilization, iron transport, and transcription regulator. Under an iron\u2010restricted medium, we experimentally found that a Fur transcription regulator (CBO1372) and two operons (DedA, CBO2610\u2013CBO2614 and ABC transporter, CBO0845\u2013CBO0847) are shown to be differentially expressed in <jats:italic>Clostridium botulinum<\/jats:italic> A ATCC 3502. This study has provided\u2010us novel insights into the diversity of Fur transcription regulators in different <jats:italic>Clostridium botulinum<\/jats:italic> strains and diversity of Fur\u2010targeted genes, as well as a better understanding of the dynamic changes in iron restriction occurring in response to this stress.<\/jats:p>","DOI":"10.1155\/2011\/934756","type":"journal-article","created":{"date-parts":[[2011,12,12]],"date-time":"2011-12-12T21:01:04Z","timestamp":1323723664000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Fur Transcription Regulator and Fur\u2010Regulated Genes in <i>Clostridium botulinum<\/i> A ATCC 3502"],"prefix":"10.1155","volume":"2011","author":[{"given":"Weibin","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junhua","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengyuan","family":"Zang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingying","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peipei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,12,12]]},"reference":[{"key":"e_1_2_5_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00078-3"},{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00103"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2190001"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-146-12-3171"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03337.x"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-725"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm631"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-010-9199-5"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/0470857897.ch8"},{"journal-title":"Current Protocols in Bioinformatics","article-title":"Multiple sequence alignment using ClustalW and ClustalX","author":"Thompson J. D.","key":"e_1_2_5_10_2"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm092"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.3844805"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.849004"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03413.x"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1419.021"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1745-6150-4-46"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02703681"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm068"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.11.6510-6525.2003"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-8-72"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.14.4865-4874.2005"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-003-0602-z"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2011\/934756.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2011\/934756.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2011\/934756","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T10:39:24Z","timestamp":1720089564000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2011\/934756"}},"subtitle":[],"editor":[{"given":"Ayman","family":"El-Kadi","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2011,1]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["10.1155\/2011\/934756"],"URL":"https:\/\/doi.org\/10.1155\/2011\/934756","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"type":"print","value":"2314-6133"},{"type":"electronic","value":"2314-6141"}],"subject":[],"published":{"date-parts":[[2011,1]]},"assertion":[{"value":"2011-08-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-10-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-12-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"934756"}}