{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T02:05:06Z","timestamp":1767665106820,"version":"3.41.2"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,7,21]],"date-time":"2011-07-21T00:00:00Z","timestamp":1311206400000},"content-version":"vor","delay-in-days":201,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["53687"],"award-info":[{"award-number":["53687"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:p>Very few studies have been performed to evaluate the effect of culture conditions on the production or activity of active metabolites in algae. Previous studies suggest that the synthesis of bioactive compounds is strongly influenced by irradiance level. To investigate whether the antiproliferative activity of <jats:italic>Udotea flabellum<\/jats:italic> extracts is modified after cultivation, this green alga was cultured under four photon flux densities (PFD) for 30 days. After 10, 20, and 30 days, algae were extracted with dichloromethane: methanol and screened for antiproliferative activity against four human cancer cell lines (laryngeal\u2014Hep\u20102, cervix\u2014HeLa, cervix squamous\u2014SiHa and nasopharynx\u2014KB) by SRB assay. Lipid and phenol content were evaluated by standardized methods on algae organic extracts. After 10 days of cultivation, organic <jats:italic>U. flabellum<\/jats:italic> extracts showed a significant increase in antiproliferative activity on Hela and SiHa cells when compared to noncultured algae extracts. Extracts obtained after 10 and 20 days of culture were active on KB and Hep\u20102 cells. Total phenol and polyunsaturated fatty acid content in organic extracts changed with cultivation time but not by irradiance treatment. Extracts from <jats:italic>U. flabellum<\/jats:italic> obtained after 10 and 20 days of culture have been selected for fractionation and isolation of active compounds.<\/jats:p>","DOI":"10.1155\/2011\/969275","type":"journal-article","created":{"date-parts":[[2011,7,21]],"date-time":"2011-07-21T19:01:21Z","timestamp":1311274881000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Enhanced Antitumoral Activity of Extracts Derived from Cultured <i>Udotea flabellum<\/i> (Chlorophyta)"],"prefix":"10.1155","volume":"2011","author":[{"given":"Rosa","family":"Moo-Puc","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Robledo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yolanda","family":"Freile-Pelegrin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,7,21]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1021\/np068054v"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041\u20100101(00)00154\u20109"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20108741(00)00341\u2010X"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1039\/b603047p"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2003.10.131"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02920141"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008113108834"},{"key":"e_1_2_7_8_2","doi-asserted-by":"crossref","first-page":"177","DOI":"10.4314\/tjpr.v10i2.66561","article-title":"Improved antitumoral activity of extracts derived from cultured Penicillus dumetosus","volume":"10","author":"Moo-Puc R.","year":"2011","journal-title":"Tropical Journal of Pharmaceutical Research"},{"key":"e_1_2_7_9_2","first-page":"151","article-title":"Research and discovery trends of Chinese medicine in the new century","volume":"15","author":"Lee K. 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