{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:26:55Z","timestamp":1790717215007,"version":"4.1.0"},"reference-count":113,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biomedicine and Biotechnology"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>Fungi comprise a vast group of microorganisms including the Ascomycota (majority of all described fungi), the Basidiomycota (mushrooms or higher fungi), and the Zygomycota and Chytridiomycota (basal or lower fungi) that produce industrially interesting secondary metabolites, such as \u03b2-lactam antibiotics. These compounds are one of the most commonly prescribed drugs world-wide. Since Fleming's initial discovery of<jats:italic>Penicillium notatum<\/jats:italic>80 years ago, the role of<jats:italic>Penicillium<\/jats:italic>as an antimicrobial source became patent. After the isolation of<jats:italic>Penicillium chrysogenum<\/jats:italic>NRRL 1951 six decades ago, classical mutagenesis and screening programs led to the development of industrial strains with increased productivity (at least three orders of magnitude). The new \u201comics\u201d era has provided the key to understand the underlying mechanisms of the industrial strain improvement process. The review of different proteomics methods applied to<jats:italic>P. chrysogenum<\/jats:italic>has revealed that industrial modification of this microorganism was a consequence of a careful rebalancing of several metabolic pathways. In addition, the secretome analysis of<jats:italic>P. chrysogenum<\/jats:italic>has opened the door to new industrial applications for this versatile filamentous fungus.<\/jats:p>","DOI":"10.1155\/2012\/105109","type":"journal-article","created":{"date-parts":[[2012,1,19]],"date-time":"2012-01-19T21:04:36Z","timestamp":1327007076000},"page":"1-15","source":"Crossref","is-referenced-by-count":59,"title":["Proteomics Shows New Faces for the Old Penicillin Producer<i>Penicillium chrysogenum<\/i>"],"prefix":"10.1155","volume":"2012","author":[{"given":"Carlos","family":"Barreiro","sequence":"first","affiliation":[{"name":"Proteomics Service of INBIOTEC, Instituto de Biotecnolog\u00eda de Le\u00f3n (INBIOTEC), Parque Cient\u00edfico de Le\u00f3n, Avenida. Real, no. 1, 24006 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan F.","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Proteomics Service of INBIOTEC, Instituto de Biotecnolog\u00eda de Le\u00f3n (INBIOTEC), Parque Cient\u00edfico de Le\u00f3n, Avenida. Real, no. 1, 24006 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Garc\u00eda-Estrada","sequence":"additional","affiliation":[{"name":"Proteomics Service of INBIOTEC, Instituto de Biotecnolog\u00eda de Le\u00f3n (INBIOTEC), Parque Cient\u00edfico de Le\u00f3n, Avenida. Real, no. 1, 24006 Le\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"98","reference":[{"issue":"3","key":"1","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1128\/CMR.12.3.454","volume":"12","year":"1999","journal-title":"Clinical Microbiology Reviews"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/S0953-7562(09)80810-1"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO.1997.87.9.888"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1017\/S0953756201004725"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.9.5544-5550.2005"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2009.03042.x"},{"key":"7","first-page":"67","volume-title":"Ascomycete relationships: dating the origin of asexual lineages with 18S ribosomal RNA gene sequence data","year":"1993"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-011-3143-4"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycres.2007.03.004"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1017\/S0953756201005196"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2010.04.008"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2008.07.001"},{"key":"13","first-page":"226","volume":"10","year":"1929","journal-title":"British Journal of Experimental Pathology"},{"issue":"6","key":"14","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1042\/bj0261907","volume":"26","year":"1932","journal-title":"Biochemical Journal"},{"key":"15","volume":"2","year":"1949"},{"issue":"1","key":"16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1600-0463.2009.02563.x","volume":"118","year":"2010","journal-title":"Acta Pathologica, Microbiologica et Immunologica Scandinavica"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2009.09.011"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-7915.2009.00123.x"},{"key":"19","first-page":"577","volume-title":"Evolution of fungal \u00df-lactam biosynthesis gene clusters","volume":"1","year":"2010"},{"issue":"3","key":"20","first-page":"130","volume":"6","year":"1953","journal-title":"The Journal of Antibiotics"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2164(08)70473-8"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1038\/183257b0"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1023\/A:1001738823146"},{"key":"24","volume-title":"Penicillins and cephalosporins","year":"2011"},{"issue":"6","key":"25","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1128\/JB.48.6.639-659.1944","volume":"48","year":"1944","journal-title":"The Journal of Bacteriology"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1946.tb31753.x"},{"issue":"1","key":"27","first-page":"152","volume":"68","year":"1946","journal-title":"Journal of the American Chemical Society"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.2307\/3755661"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1021\/ie50520a032"},{"key":"30","first-page":"97","volume-title":"Strain improvement and preservation of beta-lactam producing microorganisms","year":"1983"},{"key":"31","first-page":"270","volume":"52","year":"2002","journal-title":"SIM News"},{"key":"32","first-page":"105","volume-title":"The Panlabs Penicillium strain improvement program","year":"1986"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1656(91)90227-M"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.13.6200"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(98)01229-3"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jim.2900411"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2007.03.008"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2006.03.001"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-007-0999-4"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2008.09.004"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00077-10"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01557-10"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20080369"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20051599"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1007\/BF00334395"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01702-10"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1007\/s12223-010-0019-4"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.20.9132"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.21.14545"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.15.5062-5077.2004"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00226-06"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.19.5465-5471.2001"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2005.01.014"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2004.10.010"},{"issue":"2","key":"55","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1002\/j.1460-2075.1991.tb07971.x","volume":"10","year":"1991","journal-title":"The EMBO Journal"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2007.00228.x"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1498"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(92)90118-E"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02327-09"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbr.2010.03.006"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-75"},{"key":"62","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200107)1:7&lt;899::AID-PROT899&gt;3.0.CO;2-#"},{"key":"63","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500436"},{"key":"64","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200900301"},{"key":"65","doi-asserted-by":"publisher","DOI":"10.1186\/1477-5956-9-1"},{"key":"66","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-005-0047-9"},{"key":"67","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200701163"},{"key":"68","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-9-23"},{"key":"69","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M900327-MCP200"},{"key":"70","doi-asserted-by":"publisher","DOI":"10.1021\/pr1012812"},{"key":"71","doi-asserted-by":"publisher","DOI":"10.1186\/1477-5956-8-46"},{"key":"72","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2009.11.002"},{"key":"73","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2007.03.001"},{"key":"74","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.3.884-889.2005"},{"key":"114","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.9.2957-2966.2005"},{"key":"75","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305844"},{"key":"76","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"77","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-003-1254-2"},{"issue":"2","key":"78","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1097\/00001432-200304000-00005","volume":"16","year":"2003","journal-title":"Current Opinion in Infectious Diseases"},{"key":"79","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2006.08.004"},{"key":"80","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2007.07.001"},{"key":"81","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-97-3-0384"},{"key":"82","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.7.2.183"},{"key":"83","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.6.1147-1154.2005"},{"key":"84","doi-asserted-by":"publisher","DOI":"10.1038\/nature04300"},{"key":"85","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1282"},{"key":"86","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-008-1572-5"},{"key":"87","first-page":"76","volume-title":"Nachwachsende rohstoffe f\u00fcr die wei\u00dfe biotechnologie","year":"2006"},{"key":"88","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7799(94)90100-7"},{"key":"89","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M110.001412"},{"key":"90","doi-asserted-by":"publisher","DOI":"10.1021\/pr8003002"},{"key":"91","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.05.028"},{"key":"92","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzh037"},{"key":"93","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-105"},{"key":"94","doi-asserted-by":"publisher","DOI":"10.1093\/database\/bar001"},{"key":"95","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(98)01335-8"},{"key":"96","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03577.x"},{"key":"97","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2005.00302.x"},{"key":"98","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2617"},{"key":"99","year":"1992"},{"key":"100","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00036-07"},{"key":"101","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00036-07"},{"key":"102","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-009-0110-6"},{"key":"103","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2010.07741.x"},{"key":"104","doi-asserted-by":"publisher","DOI":"10.1007\/BF00312612"},{"key":"105","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2007.01.012"},{"key":"106","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2008.12.003"},{"issue":"1","key":"107","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/S0021-9258(18)54214-9","volume":"268","year":"1993","journal-title":"Journal of Biological Chemistry"},{"key":"108","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.63.1216"},{"key":"109","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(87)36041-0"},{"key":"111","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(88)79704-0"},{"key":"112","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.40.92"},{"key":"113","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-009-2148-8"}],"container-title":["Journal of Biomedicine and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2012\/105109.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2012\/105109.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2012\/105109.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,26]],"date-time":"2021-12-26T12:47:54Z","timestamp":1640522874000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2012\/105109\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":113,"alternative-id":["105109","105109"],"URL":"https:\/\/doi.org\/10.1155\/2012\/105109","relation":{},"ISSN":["1110-7243","1110-7251"],"issn-type":[{"value":"1110-7243","type":"print"},{"value":"1110-7251","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}