{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:32:45Z","timestamp":1782433965946,"version":"3.54.5"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>The uteri, spontaneously active or Ca<jats:sup>2+<\/jats:sup>(6\u2009mM) induced, were allowed to equilibrate, and to inhibit voltage-gated potassium (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msub><mml:mi>K<\/mml:mi><mml:mi>V<\/mml:mi><\/mml:msub><\/mml:mrow><\/mml:math>) channels 1\u2009mM 4-amino pyridine (4-AP) was applied for 15\u2009min before adding H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2009. \u2009H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>was added cumulatively: 2\u2009<jats:italic>\u03bc<\/jats:italic>M, 20\u2009<jats:italic>\u03bc<\/jats:italic>M, 200\u2009<jats:italic>\u03bc<\/jats:italic>M, 400\u2009<jats:italic>\u03bc<\/jats:italic>M, and 3\u2009mM. Average time for H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>concentrations (2, 20, 200, and 400)<jats:italic>\u03bc<\/jats:italic>M to reach its full effect was 15 min. H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>3\u2009mM had a prolonged effect and therefore was left to act for 30\u2009min. Two-way ANOVA showed significant differences in time dependency between spontaneous and Ca<jats:sup>2+<\/jats:sup>-induced rat uteri after applying 3\u2009mM H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>(type of contraction,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.0280<\/mml:mn><\/mml:math>), but not 400\u2009<jats:italic>\u03bc<\/jats:italic>M H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>(<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.9271<\/mml:mn><\/mml:math>). Our results indicate that H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>oxidises channel intracellular thiol groups and activates the channel, inducing relaxation. Cell antioxidative defence system quickly activates glutathione peroxidase (GSHPx) defence mechanism but not catalase (CAT) defence mechanism. Intracellular redox mechanisms repair the oxidised sites and again establish deactivation of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mrow><mml:msub><mml:mi>K<\/mml:mi><mml:mi>V<\/mml:mi><\/mml:msub><\/mml:mrow><\/mml:math>channels, recuperating contractility. In conclusion, our results demonstrate that<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mrow><mml:msub><mml:mi>K<\/mml:mi><mml:mi>V<\/mml:mi><\/mml:msub><\/mml:mrow><\/mml:math>channels can be altered in a time-dependent manner by reversible redox-dependent intracellular alterations.<\/jats:p>","DOI":"10.1155\/2012\/105820","type":"journal-article","created":{"date-parts":[[2012,10,24]],"date-time":"2012-10-24T21:00:46Z","timestamp":1351112446000},"page":"1-7","source":"Crossref","is-referenced-by-count":5,"title":["Reversible Oxidation of Myometrial Voltage-Gated Potassium Channels with Hydrogen Peroxide"],"prefix":"10.1155","volume":"2012","author":[{"given":"Isabella","family":"Appiah","sequence":"first","affiliation":[{"name":"Department of Physiology, Institute for Biological Research \u201cSinisa Stankovic,\u201d University of Belgrade, 11000 Belgrade, 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