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A plant expression system is attractive for protein production due to its low-cost and high-scalability nature and its ability to make appropriate posttranslational modifications. Here, we investigated the feasibility of using plants to produce two WNV detection and diagnostic reagents to address the current cost and scalability issues. We demonstrated that WNV DIII antigen and E16 monoclonal antibody are rapidly produced at high levels in two plant species and are easily purified. Furthermore, they are effective in identifying WNV and in detecting human IgM response to WNV infection. E16 mAb does not cross-react with other flaviviruses, therefore, is valuable for improving diagnostic accuracy. This study provides a proof of principle for using plants as a robust and economical system to produce diagnostic reagents for arboviruses.<\/jats:p>","DOI":"10.1155\/2012\/106783","type":"journal-article","created":{"date-parts":[[2011,12,4]],"date-time":"2011-12-04T21:00:33Z","timestamp":1323032433000},"page":"1-10","source":"Crossref","is-referenced-by-count":40,"title":["A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants"],"prefix":"10.1155","volume":"2012","author":[{"given":"Junyun","family":"He","sequence":"first","affiliation":[{"name":"The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA"},{"name":"College of Technology and Innovation, Arizona State University, Mesa, AZ 85212, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huafang","family":"Lai","sequence":"additional","affiliation":[{"name":"The Biodesign Institute, Arizona State University, Tempe, AZ 85287, 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